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48-602MAG
Buffer Detection Kit for Magnetic Beads
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612200
Sigma-AldrichTissue Plasminogen Activator, Human
Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acids. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration.
Catalogue Number
612200
Brand Family
Calbiochem®
Synonyms
tPA
References
References
Kim, Y.H., et al. 1999. Science284, 647. Tsirka, S.E., et al. 1995. Nature 377, 340. Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.
Product Information
Form
Lyophilized
Formulation
Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween®-80 Detergent.
Tissue Plasminogen Activator, Human Certificates of Analysis
Title
Lot Number
612200
References
Reference overview
Kim, Y.H., et al. 1999. Science284, 647. Tsirka, S.E., et al. 1995. Nature 377, 340. Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-May-2011 JSW
Synonyms
tPA
Description
Native tissue plasminogen activator from a human melanoma cell line. A serine protease that contains a single chain of 527 amino acid residues. Binds to fibrin via lysine binding sites at its amino terminus and activates bound plasminogen. Cleaves plasminogen to form active plasmin, the proteolytic enzyme that dissolves clots. Reported to be involved in neurite outgrowth, regeneration, and migration. Plays an important role in excitotoxin-induced neuronal degeneration. Dihydropyridine Ca2+ antagonist may increase levels of tPA.
Form
Lyophilized
Formulation
Lyophilized from 100 mM PBS, 3.5 mg/ml L-arginine, 0.001% Tween®-80 Detergent.
Source
Prepared from cell line that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Purity
≥95% by SDS-PAGE
Specific activity
≥300 KU/mg protein
Solubility
Reconstitute with diH₂O to a final concentration of 1 mg/ml (e.g., 100 µg should be reconstituted in 100 µl).
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity
Standard Handling
References
Kim, Y.H., et al. 1999. Science284, 647. Tsirka, S.E., et al. 1995. Nature 377, 340. Winther, K., et al. 1992. J. Cardiovasc. Pharmacol.19, S21.
