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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Recombinant, human TRAIL expressed in NSO cells a type II transmembrane protein with a C-terminal extracellular domain, which exhibits homology to other TNF family members. Displays potent antitumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues.
Catalogue Number
616376
Brand Family
Calbiochem®
Synonyms
Apo2L; TNF-Related Apoptosis-Inducing Ligand
References
References
Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343. Mariani, S.M., et al. 1997. J. Cell Biol.137, 221. Pan, G., et al. 1997. Science277, 815. Pan, G., et al. 1997. Science276, 111. Sheridan, J.P., et al. 1997. Science277, 818. Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤1 EU/µg TRAIL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
Canadian export regulations
Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
12-September-2008 RFH
Synonyms
Apo2L; TNF-Related Apoptosis-Inducing Ligand
Description
Recombinant, human TRAIL (amino acids 95-281) with an amino-terminal 6X histidine tag and expressed in NSO cells. A type II transmembrane protein with a C-terminal extracellular domain that exhibits homology to other members of this tumor necrosis factor (TNF) family. Displays potent anti-tumor activity against selected targets. Induces apoptosis in a number of transformed cell lines but not in normal tissues. Recombinant bioactive TRAIL is a non-disulfide-linked homotrimer but may contain a small amount (up to 10%) of disulfide-linked homodimers.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TRAIL, pH 7.4.
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: ≤1 EU/µg TRAIL
Biological activity
ED₅₀ = 4-12 ng/ml as measured by its ability to induce cytotoxicity in mouse L-929 cell line in the presence of metabolic inhibitors actinomycin D
Solubility
Reconstitute to ≥20 µg/ml in sterile PBS, 0.1% BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.
Toxicity
Standard Handling
References
Griffith, T.S., et al. 1999. J. Exp. Med.189, 1343. Mariani, S.M., et al. 1997. J. Cell Biol.137, 221. Pan, G., et al. 1997. Science277, 815. Pan, G., et al. 1997. Science276, 111. Sheridan, J.P., et al. 1997. Science277, 818. Pitti, R.M., et al. 1996. J. Biol. Chem.271, 12687.
