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654205 TNF-α, Human, Recombinant, E. coli

TNF-α, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: rhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α

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654205
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
654205-10UGCN Plastic ampoule 10 μg
Description
OverviewRecombinant, human tumor necrosis factor-α expressed in E. coli. A cytokine produced primarily by activated macrophages that activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein kinase (SAPK).
Catalogue Number654205
Brand Family Calbiochem®
SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
References
ReferencesMerck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
Product Information
CAS number94948-59-1
FormLyophilized
FormulationCarrier-free
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity≥95% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSMT6435000
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
654205-10UGCN 04055977184594

Documentation

TNF-α, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

TNF-α, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
654205

References

Reference overview
Merck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-January-2009 JSW
SynonymsrhTNF-α, rh-Cachectin, Tumor Necrosis Factor-α
DescriptionRecombinant, human tumor necrosis factor-α expressed in E. coli. Activates a variety of immune defense mechanisms by interactions with polymorphonuclear leukocytes, T cells, antibody-producing B lymphocytes, fibroblasts, and hematopoietic bone marrow cells. Activity is not species-specific. Induces apoptosis in human blood and bone marrow neutrophils and in endothelial cells. Increases the iNOS levels in vascular smooth muscle cells. Involved in pathophysiological processes of several chronic and acute diseases. Stimulates stress activated protein (SAP) kinase.
FormLyophilized
FormulationCarrier-free
CAS number94948-59-1
RTECSMT6435000
Purity≥95% by SDS-PAGE
ContaminantsEndotoxin: ≤0.1 ng/µg TNF-α
Biological activityED₅₀ = 20-50 pg/ml as measured in a cytotoxicity assay with the TNF-α-susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
SolubilityReconstitute with sterile, dH₂O to a final concentration of 0.1-1 mg/ml. Further dilutions should be made in low endotoxin medium or aqueous buffer containing carrier protein such as heat inactivated FCS or tissue culture grade BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C) for long-term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C or -70°C or up to 1 month at 4°C.
Toxicity Standard Handling
ReferencesMerck Index 13, 9882.
Tsuchida, H., et al. 1995. J. Immunol. 154, 2403.
Westwick, J.K., et al. 1995. J. Biol. Chem. 270, 22689.
Polunovsky, V.A., et al. 1994. Exp. Cell Res. 214, 584.
Koide, M., et al. 1993. FEBS Lett. 318, 213.
Vilcek, J., and Lee, T.H. 1991. J. Biol. Chem. 266, 7313.