Steriflip® Filter Units
No sample transfer, just connect your tube and filter
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Overview
The Steriflip filter unit is designed to work with standard 50 mL centrifuge tubes, which eliminates sample transfer. To filter, just attach the unit to a 50 mL centrifuge tube containing your sample, flip it over, and apply the vacuum. When finished, the filtrate collects in the attached 50 mL centrifuge tube.
New pore sizes ideal for Cell Isolation applications
Steriflip Nylon Net filters are now available in 40, 60 and 100 µm pore sizes, making them ideal for stem cell, cardiomyocite, neurological and skeletal tissue applications. The vacuum-assisted, closed system option ensures sample sterility while enabling faster separation of large volumes of cellular material and improved recoveries.
The Steriflip® filter unit eliminates messy pouring during sample transfer. Directly attach the unit to a 50 mL centrifuge tube containing your sample, flip it over, and apply vacuum. The filtrate collects in the attached 50 mL centrifuge tube. Steriflip® filters come in different pore sizes that are ideal for cell isolation. These include nylon net filters in 40, 60 and 100 µm pore sizes, which are ideal for stem cell, cardiomyocyte, neurological and skeletal tissue applications. The vacuum-assisted, closed system ensures sample sterility while enabling fast separation of large volumes of cellular material for efficient recoveries.
Features & Benefits
- Eliminates messy sample transfer via a simple vacuum-assisted, closed system
- Isolate cells from cellular debris using any of the various pore-sized nylon net filters
- Ideal for sterilizing small batches of culture media
- Attaches to any standard 50 mL centrifuge tube, eliminating unnecesary materials, time and risk of spillage
Applications
Sterilization of Aqueous Solutions (up to 50 mL), Sterile Filtration of Small Batches of Culture Media, Sterile Filtration of Buffers with High Value Protein or Small Molecule Components, Cell Isolation
Specifications
| Materials | |
| Device | MBS/Polypropylene |
| Membrane | As indicated in table |
| Inlet Fittings | Double lead thread with vacuum port |
| Outlet Fittings | Double lead thread for 50 mL centrifuge tube |
| Filtration Area | 7 cm² |
| Process Volume | 50 mL |
| Hold-up Volume | 0.6 mL |
| Maximum Operating Temperature | 45 °C |
| Sterilization Method | Gamma irradiation |
Ordering Information
Documentation
References
| Reference overview |
|---|
| Novel Single-Cell-Level Phenotypic Assay for Residual Drug Susceptibility and Reduced Replication Capacity of Drug Resistant Human Immunodeficiency Virus Type 1 Haili Zhang, Yan Zhou, Cecily Alcock, Tara Kiefer, Daphne Monie, Janet Siliciano, Quan Li, Paul Pham, Joseph Cofrancesco, Deborah Persaud, and Robert F. Siliciano; Journal of Virology, February 2004, p. 1718-1729, Vol. 78, No. 4. Journal of Virology, February 2004, p. 1718-1729, Vol. 78, No. 4. 2004 |
FAQ
| Question | Answer | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Should I filter my acrylamide solution prior to gel casting? | Yes, filtering your acrylamide solution before adding the polymerization activator ammonium persulfate and TEMED will accomplish two things. 1. It will eliminate particulate matter that may interfere with base calling when sequencing or that may create "hot" spots in the gel, and 2. It will aid in degassing, which will promote efficient polymerization of the gel. Products appropriate for this application would be Stericup, Steritop or Steriflip with express membrane. |
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| Is there a stand for the Steriflip? | Yes. If you would prefer to put the Steriflip unit into a stand when vacuum filtering your sample rather then holding it , you can order catalog number SC50BS001. | ||||||||||||||||
| While filtering serum through my Steriflip the solution appears to be bubbling what can I do? | Because Steriflip is a closed system, there is a small upstream vent that will bubble air slowly through the solution before it is filtered. If the foaming is an objection, you may purchase SC50FL025 that is a package of 25 non-sterile funnels. The funnel attaches instead of the tube, then you can pour the solution through into the funnel and turn on the vacuum. The solution will be filtered into the receiving tube. | ||||||||||||||||
| What is Steriflip? | Steriflip is a sterile vacuum-driven device for use with 50 ml centrifuge tubes. The filter unit incorporates Millipore's 0.22um Express (PES) membrane. | ||||||||||||||||
| What tubes will work with my Steriflip? | The Steriflip comes with one sterile polypropylene 50 ml tube that you filter your sample into, but you must supply your own starting tubes. The unit will fit onto most standard double start (double lead ) 50 ml tubes. Millipore sells these tubes in 100 and 500 packs.
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| What is the shelf life on the Steriflip? | The shelf life on the Steriflip is two years from the date of manufacture. The expiration date will be on the package when you receive it. | ||||||||||||||||
| What is the typical hold-up volume of the of the Steriflip? | The typical hold-up volume of the Steriflip is 600ul. | ||||||||||||||||
| Do you have a filter for 100% serum? | Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls. | ||||||||||||||||
| Is the Express membrane surfactant free? | Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight. | ||||||||||||||||
| Is the Steriflip autoclavable? | No. Steriflips are provided to you pre-sterilized and individually wrapped. The sterilization method is gamma irradiation. | ||||||||||||||||
Related Products & Applications
Product Families
In-Line Millex® Filter Units, 25 mm and 50 mm, Hydrophobic PTFEIdeal for sterilizing gases, venting sterile containers, and sterilizing or clarifying organic solutions.Learn More >> |
Millivac™ Vacuum PumpsMaintenance-free, mini-diaphragm pumpsLearn More >> |
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| Protein Purification |
| Sample Preparation |
| Dialysis and Filtration |
Related Products By: Brand Facete
| Steriflip® |
Categories
| Life Science Research > Cell Culture and Systems > Sterile Filtration Devices |
| Materials | |
| Device | MBS/Polypropylene |
| Membrane | As indicated in table |
| Inlet Fittings | Double lead thread with vacuum port |
| Outlet Fittings | Double lead thread for 50 mL centrifuge tube |
| Filtration Area | 7 cm² |
| Process Volume | 50 mL |
| Hold-up Volume | 0.6 mL |
| Maximum Operating Temperature | 45 °C |
| Sterilization Method | Gamma irradiation |