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QIA99 Soluble Tissue Plasminogen Activator ELISA Kit

QIA99
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Species ReactivityDetection Methods
HColorimetric

Products

Catalogue NumberPackaging Qty/Pack
QIA99-1KITCN Glass bottle 1 kit
Description
OverviewUseful for the quantitative detection of soluble tissue-type plasminogen activator (t-PA). Altered levels of t-PA have been implicated in the progression of Grave’s disease, liver disorders, diabetic retinopathy, cardiovascular disease, thrombosis, myocardial infarction, and stroke.
Catalogue NumberQIA99
Brand Family Calbiochem®
Application Data
Materials Required but Not Delivered 5 and 10 ml graduated pipettes
10 to 1,000 µl adjustable single channel micropipettes with disposable tips
50 to 300 µl adjustable multichannel micropipette with disposable tips
Multichannel micropipette reservoir
Beakers, flasks, and cylinders necessary for preparation of reagents
Multichannel wash bottle or automatic wash system for delivery of wash solution
Microwell strip reader capable of reading at 450 nm with 620 nm as optional reference wavelength
Glass-distilled or deionized water
Calculator with program to perform linear regression analysis
References
ReferencesLi, Y., et al. 1998. Eur. J. Clin. Invest. 28, 1050.
Skrha, J., et al. 1994. Clin. Chim. Acta. 229, 5.
Okabe, K., et al. 1992. Gastroenterol. Jpn. 27, 61.
Dano, K., et al. 1988. In Tissue-Type Plasminogen Activator (t-PA): Physiological and Clinical Aspects (Kluft, C.,
ed.) pp. 20-46, CRC Press, Boca Raton, Florida.
Kluft, C. 1988. In Tissue-Type Plasminogen Activator (t-PA): Physiological and Clincal Aspects(Kluft, C., ed.) pp. 47-49, CRC Press, Boca Raton, Florida.
Kruithof, E.K.O. 1988. In Tissue-Type Plasminogen Activator (t-PA): Physiological and Clincal Aspects (Kluft, C.,
ed.) pp. 190-210, CRC Press, Boca Raton, Florida.
Mehta, J., et al. 1987. J. Am. Coll. Cardiol. 9, 263.
Wilman, B., et al. 1986. J. Lab. Clin. Med. 105, 265.
Product Information
Detection methodColorimetric
Form96 Tests
Format96-well plate
Kit containsPre-Coated Microtiter Plate Strips, Anti-t-PAI/HRP-Conjugate, t-PA Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Sample Diluent, Substrate Solutions I and II, Stop Solution, Plate Covers, and a user protocol
Quality LevelMQ100
Biological Information
Assay range16 - 1000 pg/ml
Assay time2.5 h
Sample TypeCell culture supernatant, serum, plasma, or other biological fluids
Species Reactivity
  • Human
Physicochemical Information
Sensitivity8 pg/ml
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage +2°C to +8°C
Storage ConditionsUpon arrival store the entire contents of the kit at 4°C.
Do not freeze Yes
Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Supplemental Information
Kit containsPre-Coated Microtiter Plate Strips, Anti-t-PAI/HRP-Conjugate, t-PA Standard, Wash Buffer Concentrate, Assay Buffer Concentrate, Sample Diluent, Substrate Solutions I and II, Stop Solution, Plate Covers, and a user protocol
Global Trade Item Number
Catalogue Number GTIN
QIA99-1KITCN 04055977209266