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HTS126RTA Ready-to-Assay™ TRH Thyrotropin-Releasing Hormone Receptor Frozen Cells

Human TRH GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1 Entrez Gene Number: NM_003301
HTS126RTA
1 kit  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

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Key Spec Table

SpeciesHost CellsProtein Target
Human Chem-1TRH
Description
Catalogue NumberHTS126RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ TRH Thyrotropin-Releasing Hormone Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Thyrotropin-releasing hormone (TRH) is a tripeptide (L-pyroglutamyl-L-histidinyl-L-proline amide) that is released from the hypothalamus to stimulate release of thyroid-stimulating hormone from the thyrotropes of the anterior pituitary. In addition, TRH is synthesized in other regions of the CNS, where it appears to function as a neuromodulator to influence arousal, cognition, motor activity, depression, and food and water intake. TRH functions in the autonomic nervous system to modulate gastrointestinal motility and acid secretion (Nillni and Severino, 1999). The biological functions of TRH are mediated by binding to two Gq-coupled seven transmembrane receptors, TRH1 and TRH2 (Sun et al., 2003). TRH1 is present in humans, rodents, and other vertebrates, whereas TRH2 has been identified only in rodents. Synthetic TRH (protirelin) is used clinically to evaluate the functionality of the hypothalamic-pituitary-thyroid axis. Millipore’s cloned human TRH -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant TRH expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between TRH and its ligands.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman TRH GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host CellsChem-1
Protein TargetTRH
Target Sub-FamilyTRH
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HTS126RTA 04053252555404

Documentation

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Categories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells