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553506 RAP, Human, Recombinant, E. coli

RAP, Human, Recombinant, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Receptor Associated Protein

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553506
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
553506-50UGCN Plastic ampoule 50 μg
Description
OverviewRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Catalogue Number553506
Brand Family Calbiochem®
SynonymsReceptor Associated Protein
References
ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
Product Information
FormLiquid
Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
553506-50UGCN 04055977193664

Documentation

RAP, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

RAP, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
553506

References

Reference overview
Li, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-April-2009 JSW
SynonymsReceptor Associated Protein
DescriptionRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
FormLiquid
Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035