Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

IP04 Protein G Plus-Agarose Suspension

Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Protein G Plus-Agarose Suspension MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
IP04
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
IP04-1.5MLCN Plastic ampoule 1.5 ml
Description
OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Catalogue NumberIP04
Brand Family Calbiochem®
References
Product Information
FormLiquid slurry
FormulationIn PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsAgarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IP04-1.5MLCN 07790788053833

Documentation

Protein G Plus-Agarose Suspension SDS

Title

Safety Data Sheet (SDS) 

Protein G Plus-Agarose Suspension Certificates of Analysis

TitleLot Number
IP04
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
ApplicationImmunoprecipitation (see comments)
DescriptionProtein G PLUS agarose suspension specifically formulated for immunoprecipitation.
BackgroundProtein G PLUS-Agarose has multiple Ig binding sites genetically engineered for higher binding capacity of mouse IgG1 or IgG3 as well as IgG from rabbit, rat, and other species. Mouse IgG2a or IgG2b bind moderately to Protein G PLUS. As an alternative, use Protein A, Protein G PLUS/Protein A, or goat second-step antibodies to bridge these antibodies to Protein G PLUS. For rat IgM antibodies, use goat second-step antibodies with Protein G PLUS or Protein G PLUS/Protein A.
FormLiquid slurry
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsAgarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling

Related Products & Applications

Related Products

Catalogue Number Description
IP02 Protein A Agarose Suspension
IP05 Protein G Plus/Protein A Agarose Suspension
IP10 Protein G Plus/Protein A-Agarose

Categories

Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification
Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification