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539305 Protein G, Alkaline Phosphatase Conjugate

Protein G, Alkaline Phosphatase Conjugate MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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539305
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
539305-500UGCN Plastic ampoule 500 μg
Description
OverviewProtein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.
Catalogue Number539305
Brand Family Calbiochem®
References
Product Information
FormLiquid
FormulationIn 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application NotesDirect Immunoassay (1:2500)
Application Commentsp-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
PuritySingle band at 33.8 kDa by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539305-500UGCN 04055977269079

Documentation

Protein G, Alkaline Phosphatase Conjugate SDS

Title

Safety Data Sheet (SDS) 

Protein G, Alkaline Phosphatase Conjugate Certificates of Analysis

TitleLot Number
539305
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-April-2009 RFH
ApplicationDirect Immunoassay (1:2500)
DescriptionRecombinant protein G, a cell-surface protein of Streptococcus species, binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economical means to isolate, detect and purify antibodies produced by many animals. It is especially valued because of its ability to react with human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e., bovine goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE, and IgM. Useful for detection of primary antibodies by various immunoassay methods at an average dilution of 1:2500.
FormLiquid
FormulationIn 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.
Concentration Label Please refer to vial label for lot-specific concentration
PuritySingle band at 33.8 kDa by SDS-PAGE
Preservative≤0.1% sodium azide
Commentsp-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling