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IP02 Protein A Agarose Suspension

Protein A Agarose Suspension designed for immunoprecipitation applications

Protein A Agarose Suspension MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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IP02
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
IP02-1.5MLCN Plastic ampoule 1.5 ml
Description
OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for mouse IgG2a and IgG2b and rabbit IgG.
Catalogue NumberIP02
Brand Family Calbiochem®
References
Product Information
FormLiquid slurry
FormulationIn PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Application CommentsAgarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
IP02-1.5MLCN 04055977220209

Documentation

Protein A Agarose Suspension SDS

Title

Safety Data Sheet (SDS) 

Protein A Agarose Suspension Certificates of Analysis

TitleLot Number
IP02
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
DescriptionProtein A conjugated to agarose.
FormLiquid slurry
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsAgarose solutions are supplied ready-to-use for immunoprecipitation. Use 15 µl suspension/µg primary antibody. Protein A-Agarose is recommended for use in immunoprecipitation assays using rabbit polyclonal antibodies or unmodified mouse IgG2a or IgG2b monoclonal antibodies. Mouse IgG1 or IgG3 and all rat antibodies bind poorly to Protein A. As an alternative, use Protein G PLUS, Protein G PLUS/Protein A, or rabbit second-step antibodies to bridge these antibodies to Protein A. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling

Related Products & Applications

Related Products

Catalogue Number Description
IP04 Protein G Plus-Agarose Suspension
IP05 Protein G Plus/Protein A Agarose Suspension
IP10 Protein G Plus/Protein A-Agarose

Categories

Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification
Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification