Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

539134 Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem

Protease inhibitor cocktail III, EDTA-free for inhibiting aspartic, cysteine, and serine proteases as well as aminopeptidases in mammalian cells and tissues.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Primary Target: Aspartic, cysteine, and serine proteases as well as aminopeptidases
View Products on Sigmaaldrich.com
539134
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
539134-1MLCN Plastic ampoule 1 ml
539134-1SETCN 1 set
Description
OverviewA cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cell and tissue extracts, but is also suitable for bacterial extracts. Supplied with a data sheet.
Note: available as 1 x 1 ml or as a set of 5 x 1 ml.
Catalogue Number539134
Brand Family Calbiochem®
Application Data
References
Product Information
FormLiquid
ReversibleN
Applications
Biological Information
Primary TargetAspartic, cysteine, and serine proteases as well as aminopeptidases
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Irritant
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
539134-1MLCN 07790788051280
539134-1SETCN 07790788059750

Documentation

Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Protease Inhibitor Cocktail Set III, EDTA-Free - Calbiochem Certificates of Analysis

TitleLot Number
539134

Brochure

Title
An Introduction to Inhibitors and Their Biological Applications - 1st Edition
Bulk Product Guide

Citations

Title
  • Hofer, M.J., et al. 2010. J. Neurosci. 30, 1149.
  • Diao, A., et al. 2008. Journal of Biological Chemistry 283, 6957.
  • Lloyd-Burton, S.M., et al. 2007. Journal of Biological Chemistry 282, 9526.
  • Wittmann, B.M., et al. 2007. Cancer Research 67, 9549.
  • Horowitz, J.C., et al. 2006. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L415.
  • Mattos-Graner, R.O., et al. 2006. Journal of Bacteriology 188, 3813.
  • Unoki, M., et al. 2006. Journal of Biological Chemistry 281, 34677.
  • White, A.R., et al. 2006. journal of Biological Chemistry 281, 17670.
  • Choudhury, R., et al. 2005. Molecular Biology of the Cell 16, 3467.
  • Georgescu, S.P. et al. 2005. Molecular Endocrinology 19, 2491.
  • Han, J., et al. 2005. Journal of Biological Chemistry 280, 16383.
  • Liedtke, C.M., et al. 2005. Journal of Biological Chemistry 280, 25491.
  • Taniuchi, K., et al. 2005. Cancer Research 65, 105.
  • Abdolzade-Bavil, A., et al. 2004. Proteomics 4, 1397.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision27-February-2012 RFH
    Application Data
    DescriptionThis protease inhibitor cocktail set is a specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases. This cocktail is recommended for use with mammalian cells and tissue extracts, but is also suitable for bacterial extracts. It is provided as a single vial or as a set of 5 vials. Each vial contains 1 ml protease inhibitor cocktail solubilized in DMSO with a small amount of water.
    FormLiquid
    Recommended reaction conditionsOne ml is sufficient for approximately 20 g tissue. Alternatively, depending on the application, it can be used effectively with a dilution factor in the range of 1:100-1:2000. The optimal working concentration should be determined for each individual application. A dilution of 1:200 typically works well as a starting concentration.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Irritant
    Citation
  • Hofer, M.J., et al. 2010. J. Neurosci. 30, 1149.
  • Diao, A., et al. 2008. Journal of Biological Chemistry 283, 6957.
  • Lloyd-Burton, S.M., et al. 2007. Journal of Biological Chemistry 282, 9526.
  • Wittmann, B.M., et al. 2007. Cancer Research 67, 9549.
  • Horowitz, J.C., et al. 2006. American Journal of Physiology Lung Cellular and Molecular Physiology 290, L415.
  • Mattos-Graner, R.O., et al. 2006. Journal of Bacteriology 188, 3813.
  • Unoki, M., et al. 2006. Journal of Biological Chemistry 281, 34677.
  • White, A.R., et al. 2006. journal of Biological Chemistry 281, 17670.
  • Choudhury, R., et al. 2005. Molecular Biology of the Cell 16, 3467.
  • Georgescu, S.P. et al. 2005. Molecular Endocrinology 19, 2491.
  • Han, J., et al. 2005. Journal of Biological Chemistry 280, 16383.
  • Liedtke, C.M., et al. 2005. Journal of Biological Chemistry 280, 25491.
  • Taniuchi, K., et al. 2005. Cancer Research 65, 105.
  • Abdolzade-Bavil, A., et al. 2004. Proteomics 4, 1397.
    		•