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PS1023 PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

This PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer423/425).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PS1023
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PS1023-50ULCN Plastic ampoule 50 ul
Description
OverviewRecognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425 in HT-1080 cells treated with TGF-β1.
Catalogue NumberPS1023
Brand Family Calbiochem®
Application Data
Detection of human Smad3, phosphorylated at Ser423/425, by immunoblotting. Samples: whole cell lysate from HT-1080 cells, left untreated or treated with TGF-β1, TGFR inhibitor (SB-431542), or BMP-2, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) (Cat. No. PS1023) (1:1000) (top panel) and Anti-Smad3 (total) (bottom panel). Detection: chemiluminescence.
References
ReferencesAttisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesImmunoblotting (1:1000)
Immunoprecipitation (1:50)
Application CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.
CloneC25A9
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDo not aliquot.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PS1023-50ULCN 04055977227116

Documentation

PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) Certificates of Analysis

TitleLot Number
PS1023

References

Reference overview
Attisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-April-2009 JSW
ApplicationImmunoblotting (1:1000)
Immunoprecipitation (1:50)
Application Data
Detection of human Smad3, phosphorylated at Ser423/425, by immunoblotting. Samples: whole cell lysate from HT-1080 cells, left untreated or treated with TGF-β1, TGFR inhibitor (SB-431542), or BMP-2, as indicated by the (+) and (-). Primary antibody: PhosphoDetect™ Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9) (Cat. No. PS1023) (1:1000) (top panel) and Anti-Smad3 (total) (bottom panel). Detection: chemiluminescence.
DescriptionPurified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser423/425.
BackgroundMembers of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface to the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at carboxyl terminal serine residues (Ser465/467 on Smad2; Ser423/425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4 and translocate to the nucleus to regulate gene expression.
HostRabbit
Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 423/425 phosphorylation sites of Smad3, coupled to KLH.
CloneC25A9
IsotypeIgG
Specieshuman
FormLiquid
FormulationIn 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDo not aliquot.
Toxicity Standard Handling
ReferencesAttisano, L., et al. 2002. Science 296, 1646.
Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.
Wrana, J. 2000. Science 23, 1.