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PC635 PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb

This PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of NBS1 (pSer³⁴³).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Nibrin (pSer³⁴³) Rabbit pAb

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PC635
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC635-50ULCN Plastic ampoule 50 ul
Description
OverviewRecognizes the ~95 kDa nibrin protein phosphorylated at Ser343 in δ-irradiated lymphoblastoid cells.
Catalogue NumberPC635
Brand Family Calbiochem®
SynonymsAnti-Nibrin (pSer³⁴³) Rabbit pAb
References
ReferencesGatei, M., et al. 2000. Nat. Genet. 25, 115.
Lim, D.S., et al. 2000. Nature 404, 613.
Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
Zhong, Q., et al. 1999. Science 285, 747.
Carney, J.P., et al. 1998. Cell 93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
Varon, R., et al. 1998. Cell 93, 467.
Product Information
FormLiquid
FormulationSerum diluted in PBS.
Positive controlγ-irradiated lymphoblastoid cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (1:16,000)
Immunoblotting (1:1000)
Immunoprecipitation (1 µl/500 µg cell extract)
Application CommentsThis antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC635-50ULCN 04055977207828

Documentation

PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb Certificates of Analysis

TitleLot Number
PC635

References

Reference overview
Gatei, M., et al. 2000. Nat. Genet. 25, 115.
Lim, D.S., et al. 2000. Nature 404, 613.
Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
Zhong, Q., et al. 1999. Science 285, 747.
Carney, J.P., et al. 1998. Cell 93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
Varon, R., et al. 1998. Cell 93, 467.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision12-October-2007 RFH
SynonymsAnti-Nibrin (pSer³⁴³) Rabbit pAb
ApplicationELISA (1:16,000)
Immunoblotting (1:1000)
Immunoprecipitation (1 µl/500 µg cell extract)
DescriptionRabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser343.
BackgroundNibrin, a ~95 kDa double stranded DNA repair protein, is mutated in Nijmegen breakage syndrome (NBS). Patients with NBS exhibit chromosomal instability, resulting in hypersensitivity to irradiation and cancer predisposition. Nibrin forms a manganese-sensitive complex with hMre11 and hRAD50 that has single strand DNA endonuclease and exonuclease activities and is likely to be important for DNA excision, repair, and stability. This nibrin/Mre11/RAD50 complex is inhibited by BRCA1. The ATM (ataxia telangiectasia mutated) kinase has been shown to phosphorylate nibrin in an S-phase checkpoint pathway. Nibrin levels are elevated in testis tissue, suggesting a possible role in meiotic recombination. Nibrin may also be involved in telomere length maintenance.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
IsotypeIgG
Specieshuman
Positive controlγ-irradiated lymphoblastoid cells
FormLiquid
FormulationSerum diluted in PBS.
PreservativeNone
CommentsThis antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesGatei, M., et al. 2000. Nat. Genet. 25, 115.
Lim, D.S., et al. 2000. Nature 404, 613.
Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
Zhong, Q., et al. 1999. Science 285, 747.
Carney, J.P., et al. 1998. Cell 93, 477.
Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
Varon, R., et al. 1998. Cell 93, 467.