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14-452M PDK1 Protein, active, 250 µg

Active, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.

PDK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-452M
250 µg  Also available in 10 μg size (cat#14-452) and in bulk (cat# 14-452-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-452M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPDK1 Protein, active, 250 µg
OverviewN-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end.
References
Product Information
Presentation50mM Tris-HCl, pH 7.5, 0.15M NaCl, 0.1mM EGTA, 0.03% Brij-35, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200μM PMSF and 5% glycerol
Quality LevelMQ100
Applications
ApplicationActive, N-terminal, His6-tagged, recombinant human PDK1, amino acids 52-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman PDK1 expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • PDPK1
  • MGC20087
  • PDK1
  • PkB-like
  • PRO0461
  • MGC35290
  • hPDK1
Protein TargetPDK1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O15530 # Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
SIZE: 556 amino acids; 63152 Da
SUBCELLULAR LOCATION: Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
TISSUE SPECIFICITY: Appears to be expressed ubiquitously.
PTM: Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
SIMILARITY: SwissProt: O15530 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. & Contains 1 PH domain. & Contains 1 protein kinase domain.
Molecular Weight59kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of PDKtide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-452) and in bulk (cat# 14-452-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-452M 04053252339219

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-452 PDK1 Protein, active, 10 µg

Product Families

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases