Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

70181 NovaBlue Singles™ Competent Cells - Novagen

NovaBlue Singles Competent Cells are designed for ultimate convenience and reliability in plasmid transformation.

NovaBlue Singles™ Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
70181
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
70181-3CN Glass bottle 11 rxn
70181-4CN Glass bottle 22 rxn
Description
OverviewNovaBlue competent cells are for routine molecular cloning applications. NovaBlue is a K-12 strain derivative that offers high transformation efficiency, facilitates plasmid stability, and allows blue/white screening with appropriate plasmids.

NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-µl volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance).
Genotype:
endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number70181
Brand Family Novagen®
References
Product Information
Components
GenotypeendA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac Fʹ[proA+B+ lacIqZΔM15::Tn10] (TetR)
Guaranteed efficiencyGuaranteed efficiency: > 1.5 x 10⁸ cfu/µg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
70181-3CN 04055977258387
70181-4CN 04055977258394

Documentation

NovaBlue Singles™ Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

NovaBlue Singles™ Competent Cells - Novagen Certificates of Analysis

TitleLot Number
70181

Brochure

Title
High fidelity gene amplification
The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

Citations

Title
  • Sulene L. Chi, et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Research 67, 4716-4724.
  • Thomas S. Lendvay, et al. (2007) Compensatory paracrine mechanisms that define the urothelial response to injury in partial bladder outlet obstruction. American Journal of Physiology: Renal 293, F1147-F1156.
  • Alvaro San Millan, et al. (2007) β-Lactam resistance in Haemophilus parasuis is mediated by plasmid pB1000 bearing blaROB-1. Antimicrobial Agents and Chemotherapy 51, 2260-2264.
  • Bratislav Janjic, et al. (2006) Spontaneous CD4+ T cell responses against TRAG-3 in patients with melanoma and breast cancers. Journal of Immunology 177, 2717-2727.
  • Pernilla Larsson Birgander, et al. (2005) Nucleotide-dependent formation of catalytically competent dimers from engineered monomeric ribonucleotide reductase protein R1. Journal of Biological Chemistry 280, 14997-15003.
  • Anna B. Gilg, et al. (2005) Isolation and functional expression of an animal geranyl diphosphate synthase and its role in bark beetle pheromone biosynthesis. Proceedings of the National Academy of Sciences (USA) 102, 9760-9765.
  • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
  • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
  • Catherine Paradis-Bleau, Francois Sanschagrin and Roger C. Levesque. (2005) Peptide inhibitors of the essential cell division protein FtsA. Protein Engineering Design and Selection 18, 85-91.
  • Francois Sanschagrin and Roger C. Levesque. (2005) A specific peptide inhibitor of the class B metallo-{beta}-lactamase L-1 from Stenotrophomonas maltophilia identified using phage display. Journal of Antimicrobial Chemotherapy 55, 252-255.
  • M. Shimizu, et al. (2005) Salmon serum 22 kDa insulin-like growth factor-binding protein (IGFBP) is IGFBP-1. Journal of Endocrinology 184, 267-276.
  • Marko Tammenkoski, et al. (2005) An unusual, his-dependent family I pyrophosphatase from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 41819-41826.
  • Eric A. Grovender, et al. (2004) Single-chain antibody fragment-based Adsorbent for the extracorporeal removal of β2-microglobulin. Kidney International 65, 310-322.
  • Laura I. Katona, et al. (2004) The Fur homologue in Borrelia burgdorferi. Journal of Bacteriology 186, 6443-6456.
  • Sharmila Sivendran, et al. (2004) Two novel mutant human adenylosuccinate lyases (ASLs) associated with autism and characterization of the equivalent mutant Bacillus subtilis ASL. Journal of Biological Chemistry 279, 53789-53797.
  • Deanne M. Compaan and W. Ross Ellington. (2003) Functional consequences of a gene duplication and fusion event in an arginine kinase. 206, 1545-1556.
  • Xinxin Gao, et al. (2003) Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Research 31, e143-.
  • Yusuf Kaya and James Ofengand. (2003) A novel unanticipated type of pseudouridine synthase with homologs in bacteria, archaea, and eukarya. RNA 9, 711-721.
  • Michael B. Murphy, et al. (2003) An improved method for the in vitro evolution of aptamers and applications in protein detection and purification. Nucleic Acids Research 31, e110-.
  • Céline Pujol and James B. Bliska. (2003) The ability to replicate in macrophages Is conserved between Yersinia pestis and Yersinia pseudotuberculosis. Infection and Immunity 71, 5892-5899.
    		•

    User Protocols

    Title
    TB009 Competent Cells