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480012 MilliporeNDSB Set - Calbiochem

Contains the following non-detergent sulfobetaines: 5 g of NDSB-195, 25 g of NDSB-201, and 5 g of NDSB-256.

NDSB Set - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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Overview

Replacement Information

Products

Catalogue Number	Packaging	Qty/Pack
480012-1SETCN	Glass bottle	1 set	Add To Favorites Add To Favorites Added To My Favorites

Description
Overview	Contains the following non-detergent sulfobetaines: 5 g of NDSB-195 (Cat. No. 480001), 25 g of NDSB-201 (Cat. No. 480005), and 5 g of NDSB-256 (Cat. No. 480010). Supplied with a data sheet.
Catalogue Number	480012
Brand Family	Calbiochem®

References
References	Goldberg, M.E., et al. 1996. Folding and Design 1, 21. Goldberg, M.E., et al. 1995. Folding & Design 1, 21. Vuillard, L., et al. 1995. Biochem. J. 305, 337. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

Product Information
Form	Solid
Hygroscopic	Hygroscopic
Quality Level	MQ100

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 36/37/38 Irritating to eyes, respiratory system and skin.
S Phrase	S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Irritant
Storage	+15°C to +30°C
Hygroscopic	Hygroscopic
Do not freeze	Ok to freeze
Special Instructions	Store lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
480012-1SETCN	04055977201819

Documentation

NDSB Set - Calbiochem SDS

Title
Safety Data Sheet (SDS)

NDSB Set - Calbiochem Certificates of Analysis

Title	Lot Number
480012	Enter Lot Number

References

Reference overview
Goldberg, M.E., et al. 1996. Folding and Design 1, 21. Goldberg, M.E., et al. 1995. Folding & Design 1, 21. Vuillard, L., et al. 1995. Biochem. J. 305, 337. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	11-August-2008 RFH
Description	Non-detergent sulfobetaines (NDSBs) are zwitterionic compounds. Like zwittergents (zwitterionic detergents), NDSBs carry the sulfobetaine hydrophilic head group. However, unlike zwittergents, the hydrophobic group in NDSBs is too short for micellar formation even at concentrations as high as 1M. Hence, NDSBs do not behave like detergents. Because of their zwitterionic nature, NDSBs were first employed to neutralize electrostatic interactions without increasing the conductivity in native isoelectrofocusing gels. Recently they have found utility in various other applications including isolation of membrane proteins, purification of nuclear proteins and halophilic proteins, renaturation of chemically and thermally denatured proteins and protein crystallization. They are potentially useful in solubilization and renaturation of fusion proteins from inclusion bodies. It is hypothesized that the hydrophobic group, although short, interacts with the hydrophobic regions of the protein preventing aggregation during renaturation. Also, presumably, the contribution from the short hydrophobic groups combined with charge neutralization ability of the sulfobetaine group results in higher yields of membrane proteins. NDSBs do not interfere with enzymatic assays based on chromogenic substrates bearing nitrophenyl groups and do not inhibit the activities of β-galactosidase and alkaline phosphatase, the two enzymes tested thus far. NDSB-195 has the added advantage in that it does not absorb at 280 nm, making it compatible with protein purification procedures in which the protein concentrations are determined by absorbance measurements at 280 nm. Supplied with a data sheet.
Form	Solid
Solubility	H₂O (50 mg/ml)
Storage	+15°C to +30°C Hygroscopic
Do Not Freeze	Ok to freeze
Special Instructions	Store lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
Toxicity	Irritant
References	Goldberg, M.E., et al. 1996. Folding and Design 1, 21. Goldberg, M.E., et al. 1995. Folding & Design 1, 21. Vuillard, L., et al. 1995. Biochem. J. 305, 337. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

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