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FCMAB282F Milli-Mark™ Anti-Integrin αVβ3 Antibody, clone LM609-FITC

Detect Integrin αVβ3 using this Milli-Mark Anti-Integrin αVβ3 Antibody, clone LM609-FITC validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Integrins Gene Symbol: ITGAV, DKFZp686A08142, MSK8, CD51, VNRA UniProt Number: P06756
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FCMAB282F
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB282F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-Integrin αVβ3 Antibody, clone LM609-FITC
Alternate Names
  • Vitronectin Receptor
  • CD51/CD61
  • integrin alphaVbeta3
  • alphaVbeta3
Background InformationThe integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor, integrin alphaVbeta3, appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue (Brooks, 1994). Due to its involvement in angiogenesis, integrin alphaVbeta3 is one of the most intensely studied of the integrin receptors.
Product Information
FormatFITC
Control
  • U937 cells
PresentationPurified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect Integrin αVβ3 using this Milli-Mark Anti-Integrin αVβ3 Antibody, clone LM609-FITC validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenPurified adhesion receptor from the human melanoma cell line M21 (Cherish, D. and Spiro, R. (1987) J. Biol. Chemistry, 262(36):17703-17711).
CloneLM609
HostMouse
SpecificityAntibody recognizes Integrin αVβ3.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ITGAV
  • DKFZp686A08142
  • MSK8
  • CD51
  • VNRA
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

SIZE: 1048 amino acids; 116038 Da

SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using U937 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFlow Cytometry
Antibody dilution for cellular staining:

• Prepare an antibody working solution by diluting the primary antibody 1:5 with PBS
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments
Packaging Information
Material Size100 tests
Global Trade Item Number
Catalogue Number GTIN
FCMAB282F 04053252415371