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FCMAB193F Milli-Mark™ Anti-CD33-FITC Antibody, clone WM-54

Milli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology UniProt Number: P20138
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FCMAB193F
100 tests  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB193F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-CD33-FITC Antibody, clone WM-54
Alternate Names
  • Myeloid cell surface antigen CD33
  • Sialic acid-binding Ig-like lectin 3
  • Siglec-3
  • gp67
Background InformationCD33 is a member of the siglec family (sialic acid binding immunoglobulin-like lectins) referred to as Siglec-3 (Freeman SD, Kelm S, Barber EK and Crocker PR, 1995, Blood, 2005-2012). It is the smallest member of the siglec family having a molecular weight of 67 kDa (Peiper SC, Ashmun RA and Look TA (1988) Blood., 72: 314-321). CD33 mediates protein-carbohydrate cellular interactions in addition to protein-protein recognition (Crocker, 2002). The CD33 gene is located on chromosome 19 (Peiper, 1997). CD33 is expressed by subsets of monocytes and neutrophils. CD33 is the major antigen to differentially diagnose myeloid from lymphoid leukemias, as it is expressed on 90% of myeloid and not lymphoid leukemic cells (Andrews 1989; Griffin 1984; and Sabbath 1986). Committed hemapoietic progenitors, including multipotential stem cells (CFU-GEMM) and precursors of granulocytes and macrophages (CFU-GM) and early erythroid progenitors (BFU-E) express CD33 (Peiper, 1989 and 1997). Anti-CD33, WM-54, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD33 (Peiper, 1989).
References
Product Information
FormatFITC
Control
  • PBMCs
PresentationPurified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
Quality LevelMQ100
Applications
ApplicationMilli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.
Key Applications
  • Flow Cytometry
Biological Information
ImmunogenLeukemic cells from a patient with AML.
CloneWM-54
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityAntibody recognizes Human CD33 antigen.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein A Purfied
UniProt Number
Molecular Weight67 kDa (Peiper, 1997)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using human PBMCs
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
Packaging Information
Material Size100 tests
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
FCMAB193F 04053252289170

Documentation

Milli-Mark™ Anti-CD33-FITC Antibody, clone WM-54 SDS

Title

Safety Data Sheet (SDS) 

Milli-Mark™ Anti-CD33-FITC Antibody, clone WM-54 Certificates of Analysis

TitleLot Number
Milli-Mark Anti-CD33-FITC, clone WM-54 - 2151862 2151862
Milli-Mark Anti-CD33-FITC, clone WM-54 - 2204952 2204952
Milli-Mark Anti-CD33-FITC, clone WM-54 - NRG1761335 NRG1761335
Milli-Mark™ Anti-CD33-FITC, clone WM-54 - 3733817 3733817
Milli-Mark™ Anti-CD33-FITC, clone WM-54 -2514239 2514239
Milli-Mark™ Anti-CD33-FITC, clone WM-54 -2694547 2694547
Milli-Mark™ Anti-CD33-FITC, clone WM-54 Conjugate 3015366
Milli-Mark™ Anti-CD33-FITC, clone WM-54 - 2390568 2390568
Milli-Mark™ Anti-CD33-FITC, clone WM-54 - 2329152 2329152