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444282 MMP/TACE Substrate, FS-6 - Calbiochem

The widely used MMP substrate FS-1 is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities.

MMP/TACE Substrate, FS-6 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mca-KPLG~L-Dpa-AR-NH₂

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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
444282-1MGCN Glass bottle 1 mg
Description
OverviewThe widely used MMP substrate FS-1 (Cat. No. 03-32-5032) is modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and substrate sensitivities. Exhibits 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 when compared with FS-1 and ~2-fold higher kcat/Km towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10.
Catalogue Number444282
Brand Family Calbiochem®
SynonymsMca-KPLG~L-Dpa-AR-NH₂
References
ReferencesNeumann, U., et al. 2004. Anal. Biochem. 328, 166.
Product Information
ATP CompetitiveN
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₅₅H₈₀N₁₆O₁₆
Chemical formulaC₅₅H₈₀N₁₆O₁₆
Hygroscopic Hygroscopic
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetBroad-spectrum MMP and TACE substrate
Purity≥97% by HPLC
Physicochemical Information
Cell permeableN
Peptide SequenceMca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Protect from Light Protect from light
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
444282-1MGCN 04055977185997

Documentation

MMP/TACE Substrate, FS-6 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

MMP/TACE Substrate, FS-6 - Calbiochem Certificates of Analysis

TitleLot Number
444282

References

Reference overview
Neumann, U., et al. 2004. Anal. Biochem. 328, 166.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision28-September-2007 RFH
SynonymsMca-KPLG~L-Dpa-AR-NH₂
DescriptionThe widely used MMP substrate FS-1 (Cat. No. 03-32-5032), modified by an N-terminal lysine elongation to produce a broad-spectrum MMP and TACE substrate that displays improved water solubility and sensitivity. Exhibits a 2- to 9-fold higher specificity constant (kcat/Km) towards MMP-1, -8, -13, -14 (Km (μM)= 27.5, 5.2, and 7.9, respectively) when compared with FS-1 and ~ 2-fold higher kcat/Km (778, 400 M-1 s-1) towards TACE when compared with TACE Substrate II and III (Cat. Nos. 616402 and 616403, respectively). It has been used successfully in detecting T98G glioma cell surface-associated MMP activity and is shown to be resistant to proteolysis by human leukocyte elastase, trypsin, and ADAM-10. Excitation max: 324 nm; Emission max: 400 nm.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditionsMMP: 100 mM Tris-HCL,100 mM Nacl, 10 mM CaCl2, 0-5% (w/v) BRIJ®35 detergent, 0.1% (w/v) PEG6000, pH7.5. TACE and ADAM10: (10 mM HEPES, pH 7.5).
Chemical formulaC₅₅H₈₀N₁₆O₁₆
Peptide SequenceMca-Lys-Pro-Leu-Gly~Leu-Dpa-Ala-Arg-NH₂
Purity≥97% by HPLC
SolubilityH₂O (20 mg/ml)
Storage Protect from light
+2°C to +8°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Toxicity Standard Handling
ReferencesNeumann, U., et al. 2004. Anal. Biochem. 328, 166.