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HIFN-130K-PMX9 MILLIPLEX® Human Interferon 9-plex Premixed Panel

Simultaneously analyze up to 9 interferon biomarkers with bead-based multiplex assays using the Luminex® technology, in human serum, plasma and cell culture samples.

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HIFN-130K-PMX9
96-well plate  96 well plate
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Overview

Replacement Information
Description
Catalogue NumberHIFN-130K-PMX9
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Interferon 9-plex Premixed Panel
OverviewMILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the verification process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability).

Each MILLIPLEX® panel and kit includes:
• Quality controls (QCs) to qualify assay performance
• Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
• Optimized serum matrix to mimic native analyte environment in serum and plasma samples
• Detection antibody cocktails designed to yield consistent analyte profiles within panel

In addition each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility. The MILLIPLEX® Human Interferon Panel enables you to focus on the therapeutic potential of interferons and the modulation of interferon expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Background InformationInterferons (IFNs) are widely expressed cytokines that have potent antiviral and antiproliferative effects. These cytokines are the first line of defense against viral infections and have important roles in immunosurveillance for malignant cells. IFNs are classified as type I, II and III IFNs, based upon the target receptors to which they bind. In humans, type I IFNs belong to a multigene family consisting of multiple IFNα subtypes but only one IFNβ, IFNε, IFNκ, and IFNω. There is only one type II IFN: IFNγ. The type III IFNs include IFNλ1, λ2 and λ3, which are also known as interleukin-29 (IL-29), IL 28A and IL-28B, respectively. Functionally, the type I and type III IFNs are induced during a viral infection and involved in host defense against viruses. IFNγ (type II) is primarily involved in the allergic response, in host defense against intracellular pathogens, and in control of tumor growth. Studies of IFNs have broadened the mechanistic understanding of cell signaling, gene transcription, and innate and acquired immunity.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-assay: <10%
  • Inter-assay: <20%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze up to 9 interferon biomarkers with bead-based multiplex assays using the Luminex® technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight or two-hour incubation assay.
• This assay requires 25 µL of 1:2 diluted plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all of the Human Interferon Panel analytes in human tissue/cell lysate and culture supernatant samples and serum or plasma samples.
• The Standard, QCs, and Serum Matrix require reconstitution in Hydration Buffer provided in the kit.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • IFNα2
  • IFNβ
  • IFNγ
  • IL-28A
  • IL-28B/IFNλ3
  • IL-29
  • IFNω
  • IFNε
  • IFNGR1
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • 89-98%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities for individual cytokines, chemokines and growth factors.
Standard Curve Range
  • IFNε: 274-200,000 pg/mL
  • IFNα2: 34-25,000 pg/mL
  • IFNɣ: 4.1-3,000 pg/mL
  • IFNλ3/IL-28B: 11-8,000 pg/mL
  • IFNβ: 3.4-2,500 pg/mL
  • IFNω, IFNGR1: 14-10,000 pg/mL
  • IFNλ2/IL-28A: 25-18,000 pg/mL
  • IFNλ1/IL-29: 4.8-3,500 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
HIFN-130K-PMX9 04065269018285

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