Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

431525 L-Leucine Dehydrogenase, Bacillus cereus

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: L-LeuDH, L-Leucine:NAD+-oxidoreductase

View Products on Sigmaaldrich.com
431525
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
431525-100UCN Plastic ampoule 100 u
Description
OverviewNative L-leucine dehydrogenase from Bacillus cereus. Catalyzes the NAD+-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids.
pH optimum 10.5-10.8 (L-leucine substrate) and 9.4 (α-ketoisocaproate substrate), temperature optimum 65°C.
Catalogue Number431525
Brand Family Calbiochem®
SynonymsL-LeuDH, L-Leucine:NAD+-oxidoreductase
References
ReferencesKärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol. Biotechnol. 22, 306.
Product Information
CAS number9082-71-7
Activity≥50 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD<sup>+</sup> per min at 30°C, pH 10.7.
EC number1.4.1.9
FormLiquid
FormulationIn 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥10 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
431525-100UCN 04055977210170

Documentation

L-Leucine Dehydrogenase, Bacillus cereus SDS

Title

Safety Data Sheet (SDS) 

L-Leucine Dehydrogenase, Bacillus cereus Certificates of Analysis

TitleLot Number
431525

References

Reference overview
Kärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol. Biotechnol. 22, 306.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-August-2008 RFH
SynonymsL-LeuDH, L-Leucine:NAD+-oxidoreductase
DescriptionNative L-leucine dehydrogenase from Bacillus cereus. Purified enzyme that catalyzes the NAD-dependent conversion of L-leucine to α-ketoisocaproate. Suitable for the production of L-tertiary leucine amino acids. Has an optimal pH of 10.5-10.8 for L-leucine substrate and 9.4 for α-ketoisocaproate substrate. Has an optimal temperature of 65°C.
FormLiquid
FormulationIn 100 mM NaCl, 10 mM potassium phosphate buffer, 50% glycerol, pH 7.5.
CAS number9082-71-7
EC number1.4.1.9
Specific activity≥10 units/mg protein
Activity≥50 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will convert 1.0 µmol L-leucine to α-ketoisocaproate in the presence of NAD+ per min at 30°C, pH 10.7.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKärst, U., et al. 1986. FEMS Microbiol. Lett. 37, 335.
Schütte, H., et al. 1985. Appl. Microbiol. Biotechnol. 22, 306.