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389561 Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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389561
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
389561-100UCN Glass bottle 100 u
Description
OverviewNative hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.
Catalogue Number389561
Brand Family Calbiochem®
References
ReferencesBaker, J.R., et al. 2002. Biochem. J. 365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
Product Information
CAS number37326-33-3
Activity≥ 100 U/ampule [1 U = 1 TRU (Turbidity Reducing Unit)]
Unit of DefinitionOne TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
EC number3.2.1.35
FormWhite lyophilized powder
Quality LevelMQ100
Applications
Biological Information
Specific Activity≥2000 U/A280
Physicochemical Information
ContaminantsProtease: ≤ 0.05 units/ampule (pH 6.0)
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
389561-100UCN 07790788058128

Documentation

Hyaluronidase, Streptomyces hyaluronlyticus nov. sp. SDS

Title

Safety Data Sheet (SDS) 

Hyaluronidase, Streptomyces hyaluronlyticus nov. sp. Certificates of Analysis

TitleLot Number
389561

References

Reference overview
Baker, J.R., et al. 2002. Biochem. J. 365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-July-2009 JSW
DescriptionNative hyaluronidase from Streptomyces hyaluronlyticus nov. species. Catalyzes the degradation of hyaluronic acid. Produces δ4,5-unsaturated tetra- and hexasaccharides.

Please Note: The appearance of this product can vary from gray cottony fibers to balled up droplets with no change in quality.
FormWhite lyophilized powder
Recommended reaction conditionsAssay for Enzymatic Activity Reagent Preparation • Prepare acetate buffer: 150 mM NaCl, 20 mM sodium acetate-acetic acid buffer, pH 6.0. • Prepare hyaluronic acid solution: 50 mg purified potassium hyaluronate (containing approximately 38% glucuronic acid) is dissolved in 100 ml of the above mentioned sodium acetate buffer. • Measure acidic bovine serum albumin with a spectrophotometer at 280 nm. Adjust the total absorbance of the albumin solution to 910. Dilute to 100 ml with 0.5 N acetate buffer, pH 4.3. Adjust the pH of the albumin solution to 3.1 using 4 N HCl. Boil in a water bath for 30 min at 100°C. Adjust to 100 ml with distilled H2O. This solution is stable for 3-4 weeks at 4°C. To use this solution, dilute with 5 volumes of acetate buffer from above. Activity Protocol • For the assay, mix 0.5 ml of the enzyme solution (made with acetate buffer without NaCl) with 0.5 ml of the hyaluronic acid solution. Incubate for 30 min at 60°C. • Add 4 ml of the acidic bovine serum albumin solution. Incubate for 10 min at 60°C. • Measure the turbidity at 660 nm. Obtain a blank value by using deactivated enzyme (incubate at 100°C for 10 min). Correct the reading by subtracting the blank value. (blank value - measured value/blank value) x 2 x 2 x dilution = TRU/ml
CAS number37326-33-3
EC number3.2.1.35
ContaminantsProtease: ≤ 0.05 units/ampule (pH 6.0)
Specific activity≥2000 U/A280
Activity≥ 100 U/ampule [1 U = 1 TRU (Turbidity Reducing Unit)]
Unit definitionOne TRU (Turbidity Reducing Unit = 1 U) is defined as the amount of enzyme that will hydrolyze 50% of hyaluronic acid, determined by a 50% reduction in turbidity, in 30 min at 60°C pH 6.0.
Solubility500 mM Acetate buffer, pH 6.0 (30 U/ml) or H₂O (30 U/ml)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions prepared in water are stable for up to 2 weeks at 4°C and stock solutions prepared in 500 mM acetate buffer, pH 6.0, are stable for up to 1 month at 4°C.
Toxicity Standard Handling
Merck USA index14, 4758
ReferencesBaker, J.R., et al. 2002. Biochem. J. 365, 317.
Mogelerskii, M.S. 1964. Fed. Proc. 23, T559.