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SCC301 Human iPSC Derived AFE Progenitors

Cryopreserved human iPSC derived anterior foregut endoderm (AFE) progenitor cells that express Sox2, Pax9 and EpCAM.

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SCC301
3X10<sup>6</sup> cell/vial  
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Overview

Replacement Information
Description
Catalogue NumberSCC301
DescriptionHuman iPSC Derived AFE Progenitors
Alternate Names
  • Human Anterior Foregut Endoderm Cells
Background InformationThe anterior foregut endoderm (AFE) gives rise to therapeutically relevant cell types in tissues such as the lung, esophagus, salivary glands, thymus, parathyroid and thyroid. Our cryopreserved anterior foregut endoderm (AFE) progenitor cells were derived from integration-free human iPS cells (Cat. No. SCC271) reprogrammed using the Simplicon RNA reprogramming technology (Cat. No. SCR550) in chemically defined/serum-free culture conditions. The AFE cells are double positive for Sox2 (A,C; Sigma AB5603A4; 1:50), Pax9 (B, C; Abcam ab28538; 1:200) and approximately 20-30% are double positive for EpCAM (D, F; Sigma MAB4444; 1:500) and Pax9 (E, F).

The human iPSC derived AFE progenitors are a component of the 3dGRO™ Human Lung Organoid Culture System which can be used to differentiate human iPS cells into mature lung organoids that structurally resemble the in vivo branching airway and early alveolar structures. The 3dGRO™ Human Lung Organoid Culture System includes (all sold separately):

  • Definitive Endoderm Induction Medium (SCM302)
  • Anterior Foregut Endoderm Induction Medium I (SCM305)
  • Anterior Foregut Endoderm Induction Medium II (SCM306)
  • 3dGRO™ Lung Organoid Branching Medium (SCM307)
  • 3dGRO™ Lung Organoid Maturation Medium (SCM308)
  • Human iPSC Derived AFE Progenitors (SCC301)
References
Product Information
Applications
ApplicationCryopreserved human iPSC derived anterior foregut endoderm (AFE) progenitor cells that express Sox2, Pax9 and EpCAM.
Key Applications
  • Stem Cell Culture
  • Cell Culture
Biological Information
Cell Line Type
  • Organoid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Mycoplasma: Negative
• Genotyped by STR analysis: Pass
• Human species verified
• Negative for HPV-16, HPV-18, Hepatitis A, B, C, and HIV-1 & 2 viruses by PCR.
• Cell viability test: Pass
• Marker expression (Sox2, Pax9): Pass
• Functional test (Lung organoid differentiation): Pass
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsHuman iPSC Derived AFE Progenitors should be stored in liquid nitrogen.
Packaging Information
Material Size3X106 cell/vial
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCC301 04061842154548