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368044 Granzyme A, Human, Recombinant, E. coli

Granzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: GzmA

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368044
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
368044-10UGCN Plastic ampoule 10 μg
Description
OverviewRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. A proapoptotic enzyme that induces cell death through caspase-independent mechanism, causing single-stranded DNA nicking and other apoptotic features including nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin and activate the thrombin receptor on neuronal cells and astrocytes. It also cleaves and activates interleukin-1β. Note: The M.W. is that of a homodimer.
Catalogue Number368044
Brand Family Calbiochem®
SynonymsGzmA
References
ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
EC number3.4.21.78
FormLiquid
FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
Quality LevelMQ100
Applications
ApplicationGranzyme A, Human, Recombinant, E. coli, is a trypsin-like serine protease secreted by cytotoxic T cells and natural killer cells. Induces cell death through caspase-independent mechanism.
Biological Information
Specific Activity≥100 units/µg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
368044-10UGCN 04055977192117

Documentation

Granzyme A, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Granzyme A, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
368044

References

Reference overview
Fan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-July-2008 RFH
SynonymsGzmA
DescriptionRecombinant, human granzyme A expressed in E. coli. Granzyme A is a trypsin-like serine protease found in the cytotoxic granules and secreted by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is a pro-apoptotic enzyme that induces cell death through a caspase-independent mechanism. Granzyme A-induced apoptosis results in single-stranded DNA nicking, nuclear fragmentation, chromatin condensation, loss of mitochondrial potential, and membrane blebbing. Granzyme A is also known to bind to and cleave nucleolin, activate the thrombin receptor on neuronal cells and astrocytes, cleave and activate interleukin-1β, and cleave putative HLA-associated protein II (PHAP II). Recombinant human Granzyme A expressed in E. coli.
FormLiquid
FormulationIn 520 mM NaCl, 50 mM MES, 10% glycerol, pH 6.0.
EC number3.4.21.78
Specific activity≥100 units/µg protein
Unit definitionOne unit is defined as the amount of enzyme that hydrolyzes 1 nmol Z-Lys-SBzl per min 25°C, pH 8.0.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesFan, Z., et al. 2002. Mol. Cell. Biol. 22, 2810.
Russell, J.H., and Ley, T. J. 2002. Annu. Rev. Immunol. 20, 323.
Beresford, P.J., et al. 2001. J. Biol. Chem. 276, 43285.
Simon, M.M., and Kramer, M.D. 1994. Methods Enzymol. 244, 68.