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AP501B Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed

Detect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Species Reactivity: Mouse
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AP501B
1 mg  
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Description
Catalogue NumberAP501B
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionGoat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed
Background InformationIgG is the most abundant immunoglobulin compose of two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.
Product Information
FormatBiotin
PresentationPurified by affinity chromatography on pooled mouse IgM+IgG+IgA covalently linked to agarose. Liquid in 2.0 mL PBS/NaN3.
Quality LevelMQ100
Applications
ApplicationDetect Mouse IgM+IgG+IgA (H+L) using this Goat anti-Mouse IgM+IgG+IgA (H+L) Antibody, biotin conjugate, Species Adsorbed validated for use in ELISA & IF.
Key Applications
  • ELISA
  • Immunofluorescence
Application NotesImmunofluorescence: <=1 μg/10E6 cells
ELISA: 1:5,000-1:20,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPrepared from pooled antisera from goats hyperimmunized with mouse IgM, IgG and IgA paraproteins.
EpitopeMouse IgM, IgG, IgA
HostGoat
SpecificityThe antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.
Species Reactivity
  • Mouse
Species Reactivity NoteReacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins. Reactivity with other species has not been determined.
Antibody TypePolyclonal Antibody
Purification MethodAffinity Chromatography
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt.
Packaging Information
Material Size1 mg
Global Trade Item Number
Catalogue Number GTIN
AP501B 04053252321962