Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

109203 Giemsa's azur-eosin-methylene blue

View Products on Sigmaaldrich.com
109203
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1.09203.0025 Glass bottle 25 g
1.09203.0100 100 g
Description
Catalogue Number109203
DescriptionGiemsa's azur-eosin-methylene blue
OverviewGiemsa's azur-eosin-methylene blue - for microscopy dye, is a dry dye that is used for the preparation of a staining solution. Giemsa's staining is one of the standard procedures in histology, hematology, cytology and bacteriology. When used in hematological applications, Giemsa’s stain is frequently used in combination with other dye solutions, e. g. with May-Grünwald’s solution for Pappenheim (MGG) overview staining. The stain demonstrates various cells with their morphological features better than e.g. with H&E stain in histology. This staining solution generally stains the nuclei red, based on the molecular interaction between the Eosin Y dye and an Azure B-DNA complex. Both dyes assemble to an Eosin Y - Azure B-DNA complex and the intensity of the resulting stain depends on the content of Azure B and the ratio of Azure B : Eosin Y. Furthermore, the resulting stain can vary depending on the influence of fixation, staining times, pH-value of the solutions or buffer substances. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Bulk density520 kg/m3
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Statement(s)H302: Harmful if swallowed.
H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
Precautionary Statement(s)P261: Avoid breathing dust/ fume/ gas/ mist/ vapours/ spray.
P264: Wash skin thoroughly after handling.
P280: Wear protective gloves/ eye protection/ face protection.
P301 + P312: IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal WordWarning
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerirritant
Product Usage Statements
Storage and Shipping Information
StorageStore at +5°C to +30°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Identity (UV/VIS-Spectrum)passes test
Loss on drying (105 °C)≤ 10 %
Suitability for microscopy (Blood smear)passes test
Nucleiviolet
Lymphocytes (Plasma)blue
Monocytes (Plasma)grey blue
basophilic granulocytes (Granules)dark violet
eosinophilic granulocytes (Granules)reddish to red-brown
Neutrophilic granulocytes (Granules)red-violet
Thrombocytesviolet
Erythrocytesreddish-brownish
Blood parasites (Nuclei)lightred
Global Trade Item Number
Catalogue Number GTIN
1.09203.0025 04022536111803
1.09203.0100 04022536111810

Related Products & Applications

Alternative Products

Catalogue Number Description
109204 Giemsa's azur eosin methylene blue solution

Accessories (Primary)

Catalogue Number Description
101424 May-Grünwald's eosine-methylene blue solution modified
106009 Methanol
109468 Buffer tablets pH 7.2
111374 Buffer tablets pH 6.8

Related Products By: Application Facete

Categories

IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Other Dry Dyes & Dye Mixtures