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14-743M GCK Protein, active, 250 µg

Active, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.

GCK Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-743M
250 µg  Also available in 10 μg size (cat#14-743) and in bulk (cat# 14-743-K).
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Overview

Description
Catalogue Number14-743M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionGCK Protein, active, 250 µg
OverviewN-terminal GST- tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells.
Alternate Names
  • germinal center kinase
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST-tagged, recombinant, human GCK amino acids 1-473, expressed by baculovirus in Sf21 cells. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceRecombinant protein expressed in Sf21 cells
Specific Activity2337 U/mg, where one unit of GCK activity is defined as 1nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the serine/threonine protein kinase family. Although this kinase is found in many tissues, its expression in lymphoid follicles is restricted to the cells of germinal centre, where it may participate in B-cell differentiation. This kinase can be activated by TNF-alpha, and has been shown to specifically activate MAP kinases. This kinase is also found to interact with TNF receptor-associated factor 2 (TRAF2), which is involved in the activation of MAP3K1/MEKK1.
Gene Symbol
  • MAP4K2
  • GCK
  • RAB8IP
  • BL44
Protein TargetGCK
Purification MethodPurified using glutathione-agarose. Purity 52% by SDS-PAGE and Coomassie blue staining.
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q12851 # Enhances MAP3K1 oligomerization, which may relieve amino-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. May play a role in the regulation of vesicle targeting or fusion.
COFACTOR: Magnesium.
SIZE: 819 amino acids; 91586 Da
SUBUNIT: Interacts with TRAF2, MAP3K1 and RAB8A.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Basolateral cell membrane; Peripheral membrane protein (By similarity). Golgi apparatus membrane; Peripheral membrane protein (By similarity).
TISSUE SPECIFICITY: Highly expressed in germinal center but not mantle zone B-cells. Also expressed in lung, brain and placenta and at lower levels in other tissues examined.
SIMILARITY: SwissProt: Q12851 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CNH domain. & Contains 1 protein kinase domain.
Molecular Weight80.6 kDa.
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active GCK.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-743) and in bulk (cat# 14-743-K).
Global Trade Item Number
Catalogue Number GTIN
14-743M 04053252411946