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Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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344906
Sigma-AldrichFURA 2/AM in Solution - CAS 108964-32-5 - Calbiochem
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
27-June-2008 RFH
Description
Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (Kd = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).
Form
Liquid
Formulation
A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.
Intert gas (Yes/No)
Packaged under inert gas
CAS number
108964-32-5
Chemical formula
C₄₄H₄₇N₃O₂₄
Purity
≥95% by HPLC
Storage
Protect from light -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.
Toxicity
Irritant
References
Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292. Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226. Schwartz, Y., et al. 1992. Endocrinology131, 772. Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937. Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555. Tsien, R.Y. 1980. Biochemistry19, 2396.
