Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

102439 Eosin Y-solution 0.5% alcoholic

for microscopy

Eosin Y-solution 0.5% alcoholic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
102439
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1.02439.0500 Plastic bottle 500 ml
1.02439.2500 Plastic bottle 2.5 l
Description
Catalogue Number102439
DescriptionEosin Y-solution 0.5% alcoholic
OverviewThe Eosin Y-solution 0.5%, alcoholic - for microscopy, is a ready-to-use solution that can be used for routine staining in the hematoxylin-eosin staining process (H&E) of sample material of human origin. The H&E staining process is the most frequently used staining method for histological material. The staining mechanism behind the H&E staining is a physico-chemical process. In the first step, the positively charged nuclear dye (Mayer's hematoxylin , Cat.no.109249, or Hematoxylin solution modified acc. to Gil III, Cat.no. 105154) binds to the negatively charged phosphate groups of the nucleic acid of the cell nucleus. The nuclei will be dyed dark blue to dark violet. The second step is the counterstaining with negatively charged anionic Eosin Y, a xanthene dye. Eosin binds to the positively charged plasma proteins. Cytoplasma and intercellular substances are stained pink to red, while erythrocytes will appear with yellow to orange color.
The 500 ml bottle is sufficient for approx. 750 specimens. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 90
Quality LevelMQ400
Applications
Applicationfor microscopy
Biological Information
Physicochemical Information
Density0.8889 g/cm3 (20 °C)
Flash point19.1 °C
pH value6 - 8 (H₂O) not applicable
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H317: May cause an allergic skin reaction.
H319: Causes serious eye irritation.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P233: Keep container tightly closed.
P240: Ground and bond container and receiving equipment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
WGKWGK 2 obviously hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerhighly flammable
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 1170 , 3, II
Declaration (transport by air) IATA-DGRUN 1170 , 3, II
Declaration (transport by sea) IMDG-CodeUN 1170 , 3, II
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
1.02439.0500 04027269147112
1.02439.2500 04027269147129

Related Products & Applications

Alternative Products

Catalogue Number Description
109844 Eosin Y-solution 0.5% aqueous

Related Products

Catalogue Number Description
109844 Eosin Y-solution 0.5% aqueous

Accessories (Primary)

Catalogue Number Description
100974 Ethanol
100983 Ethanol
107961 Entellan™ new
109016 Neo-Mount™
109249 Mayer's hemalum solution
109843 Neo-Clear™

Related Products By: Application Facete

Counter Staining
H & E
Histological Staining
Tissue Staining

Categories

IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Solutions