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ES010-50ML DAB Dilution Buffer 10x Concentrate, for use with ES005

50 mL DAB Dilution Buffer 10x Concentrate, for use with ES005 for Western Blotting & IHC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Western Blotting, Immunohistochemistry
ES010-50ML
50 mL  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
WB, IHC
Description
Catalogue NumberES010-50ML
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionDAB Dilution Buffer 10x Concentrate, for use with ES005
OverviewSince first introduced by Graham and Karnovsky (1966), numerous procedures for its use in histochemistry, immunohistochemistry, Western and dot blots have been described (Lewis & Knight, 1977; Larsson, 1988; Gallyas et al., 1982). In the presence of horseradish peroxidase and hydrogen peroxide, DAB oxidatively polymerizes to an insoluble brown polymer. The color can be modified and intensified by treatment with metal salts of silver, copper, nickel, cobalt and osmium (Hsu & Soban, 1982). DAB is a suspected carcinogen and must be handled with caution. CHEMICON supplies DAB as a stable liquid concentrate eliminating the necessity of handling a potentially hazardous material. The stabilization system is unique and prevents formation of partially oxidized DAB excluding nonspecific binding to other heme containing proteins so often observed with powdered DAB preparations. The concentrate can be diluted in appropriate peroxide containing buffers providing the researcher with the capability of formulating any of the numerous published DAB reaction systems. The most common procedure suggests diluting the DAB (Catalog Number ES005) 1:50 in a 100 μmol/L Tris-HCl buffer, pH 7.6, and adding H(2)O(2), to a final concentration of 0.01%. Incubation times range from 5-15 minutes. Prolonged reaction time will result in non-specific staining of other heme proteins.
Product Information
PresentationTRIS-Citrate buffer containing Hydrogen Peroxide and 0.2% Tween.
Applications
Application50 mL DAB Dilution Buffer 10x Concentrate, for use with ES005 for Western Blotting & IHC.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesDILUTION INSTRUCTIONS:

To 9 mL of deionized water add 1 mL of ES010 and 0.1 mL H2O2. This can then be used to dilute ES005. No preservatives added.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C for up to 12 months.
Packaging Information
Material Size50 mL
Global Trade Item Number
Catalogue Number GTIN
ES010-50ML 04053252286827