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SCR029 Cryopreserved Mouse Cortical Neural Stem Cells

The Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Stem Cell Culture
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SCR029
1 vial  1 x 106 cells
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Overview

Replacement Information

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCR029
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionCryopreserved Mouse Cortical Neural Stem Cells
OverviewMillipore's Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice. These primary neural stem cells may be used for a variety of research applications including drug development, for studies of neurotoxicity, neurogenesis, electrophysiology, neurotransmitter and receptor functions and CNS diseases and disorders.

We recommend that Millipore's Cryopreserved Mouse Cortical Neural Stem Cells (Catalog No. SCR029) be used in conjunction with the Neural Stem Cell Marker Characterization Kit (Catalog No. SCR019) and differentiation assays that demonstrate multipotentiality of the starting cell population.

For Research Use Only; not for use in diagnostic procedure.
Materials Required but Not Delivered1. Neural Stem Cell Expansion Medium (Millipore Catalog No. SCM003)

2. Basic fibroblast growth factor (bFGF, FGF-2; Specific Activity

> 2 x 10e6 Units/mg, Millipore Catalog No. GF003)

3. Epidermal Growth Factor (EGF; Specific Activity > 1 x 10e7 Units/mg; Millipore Catalog No. GF001)

4. Heparin (Sigma Catalog No. H-1027)

5. Poly-L-ornithine (Sigma Catalog No. P3655)

6. Laminin (Millipore Catalog No. CC095)

7. ESGRO Complete™ Accutase™ (Millipore Catalog No. SF006)

8. Tissue culture-ware

9. Phosphate-Buffered Saline (1X PBS) (Millipore Catalog No. BSS-1005-B)

10. Fixative (e.g. 4% Paraformaldehyde in 1X PBS)

11. Blocking Solution (5% normal donkey serum, 0.3% Triton X-100 in 1X PBS)

12. Primary and secondary antibodies

13. 4'-6-Diamidino-2-phenylindole (DAPI) / PBS solution

14. Anti-fading mounting solution (DABCO/PVA)

15. Hemacytometer

16. Microscope
References
Product Information
Components
  • 1x10e6 viable Mouse Cortical Neural Stem Cells: (Part No. 2004115) derived from mixed gender C57/BL6 embryonic day 15-18 mice, cryopreserved. Store in liquid nitrogen.
Quality LevelMQ100
Applications
ApplicationThe Cryopreserved Mouse Cortical Neural Stem Cells provides ready-to use primary neural stem cells isolated from the cortices of embryonic day 15-18 (E15-E18) C57/BL6 mice.
Key Applications
  • Stem Cell Culture
Biological Information
Stem Cell Type
  • Neural Stem Cells
Cell Line Type
  • Neural Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsWhen stored at the recommended storage conditions (liquid nitrogen), mouse cortical neural stem cells are stable up to the expiration date. Do not expose to elevated temperatures. Discard any remaining reagents after the expiration date. We recommend that the cells be used within ten passages.
Packaging Information
Material Size1 vial
Material Package1 x 106 cells
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR029 04053252723667