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233155 Cleland’s Reagent - CAS 3483-12-3 - Calbiochem

A protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations. Oxidized dithiothreitol: ≤0.5%.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Dithiothreitol, DTT

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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
3483-12-3C₄H₁₀O₂S₂

Products

Catalogue NumberPackaging Qty/Pack
233155-100GMCN Glass bottle 100 gm
233155-10GMCN Plastic ampoule 10 gm
233155-25GMCN Glass bottle 25 gm
Description
OverviewA protective agent for SH groups. Cyclizes as it reduces disulfides to thiols, so reaction is "driven" to completion. Does not contain waxy surfactant found in many other preparations.
Oxidized dithiothreitol: ≤0.5%.
Catalogue Number233155
Brand Family Calbiochem®
SynonymsDithiothreitol, DTT
References
ReferencesGailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.
Product Information
CAS number3483-12-3
FormWhite to off-white powder
Hill FormulaC₄H₁₀O₂S₂
Chemical formulaC₄H₁₀O₂S₂
Quality LevelMQ100
Applications
Biological Information
Purity≥97% by titration
Physicochemical Information
ContaminantsHeavy metals: ≤1 ppm
Heavy metals<1 ppm
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSEK1610000
Safety Information
R PhraseR: 22-36/38

Harmful if swallowed.
Irritating to eyes and skin.
S PhraseS: 36-26

Wear suitable protective clothing.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Harmful
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
233155-100GMCN 07790788054274
233155-10GMCN 04055977217698
233155-25GMCN 04055977217711

Documentation

Cleland’s Reagent - CAS 3483-12-3 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

Cleland’s Reagent - CAS 3483-12-3 - Calbiochem Certificates of Analysis

TitleLot Number
233155

References

Reference overview
Gailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.

Brochure

Title
Bulk Product Guide
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision29-April-2008 RFH
SynonymsDithiothreitol, DTT
DescriptionDTT is a water-soluble protective reagent for sulfhydryl groups. It maintains monothiols in the reduced state and reduces disulfides quantitatively. Most widely used for the study of disulfide exchange reactions of protein disulfides. DTT readily permeates cell membranes. Protects protein sulfhydryls and restores enzyme activity lost by the oxidation of sulfhydryl groups in vitro. As a reducing agent, it cyclizes as it reduces disulfides to thiols, so the reaction is "driven" to completion without using large excesses of reductant. If free thiols are to be assayed later, excess reductant must be removed by dialysis or some other appropriate method. Alternatively, an immobilized form of DTT (REDUCTACRYL™ Reagent, Cat. No. 233157) can be used. Oxidized dithiothreitol: ≤0.5%.
FormWhite to off-white powder
Intert gas (Yes/No) Packaged under inert gas
CAS number3483-12-3
RTECSEK1610000
Chemical formulaC₄H₁₀O₂S₂
Purity≥97% by titration
ContaminantsHeavy metals: ≤1 ppm
SolubilityEthanol or H₂O (30 mg/ml)
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot, purge with inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Harmful
Merck USA index14, 3376
ReferencesGailis, L. 1994. Eur. J. Pharmacol. 292, 39.
Lauriault, V.V.M., and O'Brien, P.J. 1991. Mol. Pharmacol. 40, 125.
Klonne, D.R., and Johnson, D.R. 1988. Toxicol. Lett. 42, 199.
Zahler, W.L., and Cleland, W.W. 1968. J. Biol. Chem. 243, 716.
Cleland, W.W. 1964. Biochemistry 3, 480.