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218783 Caspase-1, Human, Recombinant, E. coli

Caspase-1, Human, Recombinant, is expressed in E. coli and is fused at the N-terminus to a His•Tag® sequence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: IL-1β Converting Enzyme, ICE

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218783
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
218783-3000UCN Plastic ampoule 3000 u
Description
OverviewRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
Catalogue Number218783
Brand Family Calbiochem®
SynonymsIL-1β Converting Enzyme, ICE
References
ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.
Product Information
Activity≥50,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=400025">Cat. No. 400025</a>) per min at 30°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥25,000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
218783-3000UCN 04055977218756

Documentation

Caspase-1, Human, Recombinant, E. coli SDS

Title

Safety Data Sheet (SDS) 

Caspase-1, Human, Recombinant, E. coli Certificates of Analysis

TitleLot Number
218783

References

Reference overview
Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure

Citations

Title
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-March-2009 JSW
    SynonymsIL-1β Converting Enzyme, ICE
    DescriptionRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
    FormLiquid
    FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
    Purity≥90% by SDS-PAGE
    Specific activity≥25,000 units/mg protein
    Activity≥50,000 units/ml
    Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.
    Storage Avoid freeze/thaw
    ≤ -70°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
    Thornberry, N.A., et al. 1992. Nature 356, 768.
    Citation
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
    		•