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100068 BOLTON broth (base)

acc. ISO 10272 GranuCult® prime

BOLTON broth (base) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ph Value: 7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving) Solubility: 27.6 g/l
100068
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Overview

Key Spec Table

ph ValueSolubility
7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving)27.6 g/l

Products

Catalogue NumberPackaging Qty/Pack
1.00068.0500 Plastic bottle 500 g
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Description
Catalogue Number100068
OverviewGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
Product Information
HS Code3821 00 00
Quality LevelMQ200
Physicochemical Information
pH value7.2 - 7.6 (27.6 g/l, H₂O, 25 °C) (after autoclaving)
Solubility27.6 g/l
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H317: May cause an allergic skin reaction.
H318: Causes serious eye damage.
Precautionary Statement(s)P261: Avoid breathing dust.
P272: Contaminated work clothing should not be allowed out of the workplace.
P280: Wear protective gloves/ eye protection/ face protection.
P302 + P352: IF ON SKIN: Wash with plenty of water.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P333 + P313: If skin irritation or rash occurs: Get medical advice/ attention.
Signal WordDanger
Storage class10 - 13 Other liquids and solids
WGKWGK 3 highly hazardous to water
Disposal3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety Information
Categories of dangerirritant
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Specifications
Appearance (color)dark red to black
pH-value (25 °C)7.2 - 7.6
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Campylobacter, E.coli and P.mirabilis.
Additionaly monoculture of E.coli and P.mirabilis.
Afterwards confirmation on mCCDA resp. Tryptic soy agar.
Inoculum on reference medium (Campylobacter coli ATCC 43478 (WDCM 00004))≤ 100
Inoculum on reference medium (Campylobacter jejuni ATCC 33291 (WDCM 00005))≤ 100
Inoculum on reference medium (Campylobacter jejuni ATCC 29428 (WDCM 00156))≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 10000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))≥ 10000
Inoculum on reference medium (Proteus mirabilis ATCC 29906 (WDCM 00023))≥ 10000
Growth on mCCDA (Campylobacter coli ATCC 43478 (WDCM 00004))> 10 CFU
Growth on mCCDA (Campylobacter jejuni ATCC 33291 (WDCM 00005))> 10 CFU
Growth on mCCDA (Campylobacter jejuni ATCC 29428 (WDCM 00156))> 10 CFU
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013))no growth
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012))no growth
Growth on Tryptic soy agar (Proteus mirabilis ATCC 29906 (WDCM 00023))no growth
Colony color (Campylobacter coli ATCC 43478 (WDCM 00004))greyish
Colony color (Campylobacter jejuni ATCC 33291 (WDCM 00005))greyish
Colony color (Campylobacter jejuni ATCC 29428 (WDCM 00156))greyish
Incubation:
Bolton broth: 5 ± 1 hours at 37 ± 1 °C, afterwards 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C
Confirmation on mCCDA: at least 44 ± 4 hours at 41.5 ± 1 °C, Anaerocult C
Confirmation on Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C, aerobic
Global Trade Item Number
Catalogue Number GTIN
1.00068.0500 04022536977331