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391334 BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: N,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
10191-18-1C₆H₁₅NO₅S

Products

Catalogue NumberPackaging Qty/Pack
391334-100GMCN Glass bottle 100 gm
391334-25GMCN Plastic ampoule 25 gm
Description
OverviewBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
Absorbance (100 mM, 260 nm):≤0.05.
Catalogue Number391334
Brand Family Calbiochem®
SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
References
ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
Product Information
CAS number10191-18-1
FormWhite solid
Hill FormulaC₆H₁₅NO₅S
Chemical formulaC₆H₁₅NO₅S
PKA7.12 at 20°C
Quality LevelMQ100
Applications
Biological Information
Purity≥99% by titration (dry basis)
Physicochemical Information
Heavy metals≤1 ppm
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
RTECSKI7363500
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
391334-100GMCN 07790788058364
391334-25GMCN 04055977190083

Documentation

BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem SDS

Title

Safety Data Sheet (SDS) 

BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem Certificates of Analysis

TitleLot Number
391334

References

Reference overview
De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision14-July-2008 RFH
SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
DescriptionBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
FormWhite solid
CAS number10191-18-1
RTECSKI7363500
Chemical formulaC₆H₁₅NO₅S
Purity≥99% by titration (dry basis)
SolubilityH₂O (1 mg/ml)
Storage +15°C to +30°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
Toxicity Standard Handling
ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.