Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

OP01A Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate

This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate is validated for use in Immunoprecipitation, Other Applications for the detection of v-H-Ras (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
OP01A
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP01A-0.5MLCN Plastic ampoule .5 ml
Description
OverviewRecognizes the ~21 kDa ras protein in SW480 and Y1 cells and normal skin tissue. For neutralization studies, Cat. No. OP01L is recommended. For immunoprecipitation, Cat. No. OP01A is recommended.
Catalogue NumberOP01A
Brand Family Calbiochem®
References
ReferencesShimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid suspension
FormulationIn PBS.
Positive controlSW 480 or Y1 cells or normal skin tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (15 µl beads or 1 µg antibody per sample, see comments)
Other Applications (see comments)
Application CommentsAgarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras (see application references) and reacts with the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant p21 Ras
CloneY13-259
HostRat
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP01A-0.5MLCN 04055977209556

Documentation

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate Certificates of Analysis

TitleLot Number
OP01A

References

Reference overview
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.

Citations

Title
  • Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision01-October-2007 RFH
    ApplicationImmunoprecipitation (15 µl beads or 1 µg antibody per sample, see comments)
    Other Applications (see comments)
    DescriptionRat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Conjugated to agarose. Recognizes the ~21 kDa ras protein (see comments).
    BackgroundOncogene sequences originally isolated from Harvey sarcoma virus v-H-ras and Kirsten sarcoma virus v-K-ras, both encode 21,000 dalton phosphoproteins (p21's) with guanine nucleotide binding activity. Human cellular homologues of v-H-ras and v-K-ras, designated c-H-ras and c-K-ras, respectively, and a related human oncogene, c-N-ras, each encode 21,000 dalton protein analogues to the gene products of their viral counterparts.
    HostRat
    Immunogenrecombinant p21 Ras
    CloneY13-259
    IsotypeIgG₁
    Specieshuman, mouse, rat
    Positive controlSW 480 or Y1 cells or normal skin tissue
    FormLiquid suspension
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsAgarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras (see application references) and reacts with the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesShimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
    Taparowsky, E., et al. 1983. Cell 34, 581.
    Ellis, R.W., et al. 1981. Nature 292, 506.
    Shih, T.Y., et al. 1980. Nature 287, 686.
    Citation
  • Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
    		•
    Application referencesImmunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.