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07-463-S Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size

Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size/ Pack is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H4A, H4/J, HIST1H4F, HIST1H4L, H4FN, H4FA, H4FH, HIST1H4H, H4F2, H4FM, H4/A, H4FK, H4FG, HIST2H4, H4FB, H4/K, HIST1H4I, H4/N, HIST1H4B, H4FE, H4/M, H4/a, HIST1H4E, HIST4H4, HIST2H4A, H4FC, H4FI, H4/H, HIST1H4C, H4/G, HIST1H4K, H4FJ, H4FD, H4/I, H4/B, H4/C, H4/D, HIST1H4J, HIST1H4D, H4/E UniProt Number: P62805
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07-463-S
10 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RIP, WB, DB, ChIPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-463-S
DescriptionAnti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size
Alternate Names
  • H4K20me3
  • Histone H4 (trimethyl K20)
  • H4 histone family, member A
  • Histone 1, H4a
  • histone cluster 1, H4a
Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli, including phosphorylation on serine and threonine residues and methylation on lysine residues. PTMs produce configural changes in histone proteins that may induce nucleosome remodeling and expose or hide DNA sequences from transcriptional complexes. Histone H4 lysine 20 (H4K20) may undergo mono-, di-, or trimethylation, which is catalyzed by the methyltransferase PR-Set7 (Set8 or KMT5a). Methylated H4K20 plays a role in regulating DNA damage responses, mitosis, DNA replication, and gene expression. Trimethylation of H4K20 contributes to gene silencing, and is a mark of the repressive heterochromatin state.
References
Product Information
FormatAffinity Purified
Control
  • HeLa acid extract and various recombinant Histone proteins.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size/ Pack is a rabbit polyclonal antibody for detection of trimethyl-Histone H4 (Lys20) also known as H4K20me3, Histone H4 (tri methyl K20) & has been validated in IP, WB, ChIP, DB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
  • Dot Blot
  • Chromatin Immunoprecipitation (ChIP)
Application NotesWestern Blot Analysis: 2 µg/mL from a representative lot detected trimethyl-Histone H4 (Lys20) in 10 µg of BALB3T3/A31 acid extract and RAT2 acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see tables) were probed with Anti-trimethyl-Histone H4 (Lys20) (4 µg/mL).

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated Histone H4 using chromatin from HeLa cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated trimethyl-Histone H4 (Lys20) from HeLa cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H4 trimethylated at Lys20.
EpitopeTrimethylated Lys20
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes the N-terminus of Histone H4 trimethylated at Lys20.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H4A
  • H4/J
  • HIST1H4F
  • HIST1H4L
  • H4FN
  • H4FA
  • H4FH
  • HIST1H4H
  • H4F2
  • H4FM
  • H4/A
  • H4FK
  • H4FG
  • HIST2H4
  • H4FB
  • H4/K
  • HIST1H4I
  • H4/N
  • HIST1H4B
  • H4FE
  • H4/M
  • H4/a
  • HIST1H4E
  • HIST4H4
  • HIST2H4A
  • H4FC
  • H4FI
  • H4/H
  • HIST1H4C
  • H4/G
  • HIST1H4K
  • H4FJ
  • H4FD
  • H4/I
  • H4/B
  • H4/C
  • H4/D
  • HIST1H4J
  • HIST1H4D
  • H4/E
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~13 kDa observed. An uncharacterized band may appear at ~110 kDa in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa acid extract and various recombinant Histone proteins.

Western Blot Analysis: 2 µg/mL of this antibody detected trimethyl-Histone H4 (Lys20) in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-463-S 04053252907500

Documentation

Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size SDS

Title

Safety Data Sheet (SDS) 

Anti-trimethyl-Histone H4 (Lys20) Antibody, Trial Size Certificates of Analysis

TitleLot Number
Anti-trimethyl Histone H4 (Lys20), -2702117 2702117
Anti-trimethyl Histone H4 (Lys20), Trial Size - 2420549 2420549
Anti-trimethyl Histone H4 (Lys20), Trial Size - 3319400 3319400
Anti-trimethyl Histone H4 (Lys20), Trial Size - 3506459 3506459
Anti-trimethyl Histone H4 (Lys20), Trial Size - 3756558 3756558
Anti-trimethyl Histone H4 (Lys20), Trial Size - Q2243191 Q2243191
Anti-trimethyl Histone H4 (Lys20), Trial Size -2596509 2596509
Anti-trimethyl Histone H4 (Lys20), Trial Size -2617691 2617691
Anti-trimethyl Histone H4 (Lys20), Trial Size -2623694 2623694
Anti-trimethyl Histone H4 (Lys20), Trial Size Polyclonal Antibody 2972040

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies