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MABN110 Anti-pro-BDNF Antibody, clone 5D4.1

This Anti-pro-BDNF Antibody, clone 5D4.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of pro-BDNF.

Anti-pro-BDNF Antibody, clone 5D4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: BDNF UniProt Number: P23560
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MABN110
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN110
DescriptionAnti-pro-BDNF Antibody, clone 5D4.1
Alternate Names
  • Brain-derived neurotrophic factor
  • Abrineurin
Background InformationBrain Derived Neurotrophic Factor (BDNF) is a secreted protein that promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. It is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. It is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of this gene is reduced in both Alzheimer’s and Huntington disease patients. BDNF may play a role in the regulation of stress response and in the biology of mood disorders. BDNF exists as monomers or homodimers and binds to TRKB/NTRK2 receptors.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-pro-BDNF Antibody, clone 5D4.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of pro-BDNF.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: A 0.5 µg/mL dilution from a representative lot detected 10 µg of recombinant protein Pro-BDNF but not recombinant protein BDNF.
Immunohistochemistry Analysis: A 1:2000 dilution from a representative lot detected Pro-BDNF in human cerebral cortex and thalamic tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to Human pro-BDNF.
Clone5D4.1
Concentration1 mg/mL
HostMouse
SpecificityThe antibody reacts to the pro form of BDNF
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BDNF
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~ 34 kDa observed (Neurochemical Journal, 2012, Vol. 6, No. 4, pp 260-264).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat Brain cell lysate.

Western Blotting Analysis: A 0.5 µg/mL diultion of this antibody detected pro-BDNF in 200 µg of Rat Brain cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN110 04055977112719

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies