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07-030-AF555 Sigma-AldrichAnti-dimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate

This Anti-dimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FB, HIST1H3C, H3FC, HIST1H3D, H3FD, HIST1H3E, HIST1H3F, H3FF, HIST1H3G, HIST1H3H, H3FH, HIST1H3I, H3FI, HIST1H3J, H3FJ, H3FK, HIST3H3, H3FT, H3T UniProt Number: Q16695

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, T	ICC	Rb	AlexaFluor®555	Polyclonal Antibody

Description
Catalogue Number	07-030-AF555
Description	Anti-dimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate
Alternate Names	H3K4me2 H3.3B, Lys4 dimethylated H3 histone, family 3A, Lys4 dimethylated H3 histone, family 3B, Lys4 dimethylated Histone H3, Lys4 dimethylated
Background Information	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.

Product Information
Format	AlexaFluor®555
Presentation	Purified rabbit polyclonal antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Quality Level	MQ100

Applications
Application	This Anti-dimethyl Histone H3 (Lys4) Antibody, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of dimethyl Histone H3.
Key Applications	Immunocytochemistry
Application Notes	The unconjugated antiserum (Cat. No. 07-030) is shown to be suitable also for dot blot, chromatin immunoprecipitation (ChIP), ChIP-seq, immunocytochemistry, immunofluorescence, and Western blotting applications.

Biological Information
Immunogen	KLH-conjugated synthetic peptide (ART[Kme2]QTAR-GC) corresponding to amino acids 1-8 of human histone H3.
Epitope	H3K4me2
Concentration	Please refer to lot specific datasheet.
Host	Rabbit
Specificity	Specificity of this polyclonal antibody toward histone H3 Lys4 dimethylation was demonstrated by dot blot analysis using the unconjugated antiserum (Cat. No. 07-030).
Species Reactivity	Human Tetrahymena
Species Reactivity Note	Human, Tetrahymena. Broad species cross-reactivity is expected.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_003484.1
Gene Symbol	HIST1H3A H3FA HIST1H3B H3FB HIST1H3C H3FC HIST1H3D H3FD HIST1H3E HIST1H3F H3FF HIST1H3G HIST1H3H H3FH HIST1H3I H3FI HIST1H3J H3FJ H3FK HIST3H3 H3FT H3T
Modifications	Methylation
Purification Method	Protein A purified.
UniProt Number	Q16695
Molecular Weight	~17 kDa observed.

Product Usage Statements
Quality Assurance	Evaluated by Immunocytochemistry in A431 cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclear histone H3 Lys4 dimethylation in A431 cells.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µL

Global Trade Item Number
Catalogue Number	GTIN
07-030-AF555	04055977355574

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