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CBL270-I Anti-alpha-Tubulin Antibody, clone YOL1/34

This rat monoclonal Anti-alpha-Tubulin Antibody, clone YOL1/34, Cat. No. CBL270-I is validated for use in Affinity Binding Assay, Electron Microscopy, ELISA, Immunocytochemistry, and Western Blotting for the detection of alpha-tubulin.

Anti-alpha-Tubulin Antibody, clone YOL1/34 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: TUB1 UniProt Number: P09733
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CBL270-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, Porcine, R, YeastABA, EM, ELISA, ICC, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL270-I
ReplacesCBL270
DescriptionAnti-alpha-Tubulin Antibody, clone YOL1/34
Alternate Names
  • Tubulin alpha-1 chain
  • alpha-tubulin
Background InformationTubulin alpha-1 chain (UniProt P09733; also known as alpha-tubulin) is encoded by the TUB1 gene (Gene ID 854889) in yeast. Microtubules are dynamic protein filaments involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Microtubules are assembled from α- and β-tubulin subunits, both of which are subjected to various post-translational modifications (PTMs), including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation. These PTMs can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins. Because the C-terminus tyrosine/phenylalanine is preceded by glutamate (E) residues, the detyrosinated/dephenylalaninated α-tubulin is also referred to as Glu-tubulin. While tyrosine can be reattached to the C-terminus of the Glu-tubulin by tubulin-tyrosine ligase in mammals, phenylalanine is not added posttranslationally to the Glu-tubulin in yeast despite the presence of the yeast YBR094w gene that exhibits significant homology to tubulin-tyrosine ligase in other organisms
References
Product Information
FormatPurified
PresentationPurified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal Anti-alpha-Tubulin Antibody, clone YOL1/34, Cat. No. CBL270-I is validated for use in Affinity Binding Assay, Electron Microscopy, ELISA, Immunocytochemistry, and Western Blotting for the detection of alpha-tubulin.
Key Applications
  • Affinity Binding Assay
  • Electron Microscopy
  • ELISA
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected alpha-tubulin in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL from a representative lot detected alpha-tubulin in 10 µg of NIH/3T3 mouse fibroblast and PC12 rat pheochromocytoma cell lysates.

Affinity Binding Assay: Pig brain α-tubulin, α-tubulin/β-tubulin heterodimer, as well as α-tubulin proteolytic fragments a.a. 414-425 and a.a. 403-422, but not β-tubulin or actin, effectively competed against immobilized α-tubulin for YOL 1/34 binding (Breitling, F., and Little, M. (1986). J. Biol. Chem. 189(2):367-370).

Electron Microscopy Analysis: A representative lot immunostained microtubules of crude yeast nuclear envelope preparation (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).

ELISA Analysis: A representative lot detected pig brain α-tubulin by direct (non-sandwich) ELISA (Breitling, F., and Little, M. (1986). J. Biol. Chem. 189(2):367-370).

Immunocytochemistry Analysis: A representative lot immunostained fixed NIH/3T3 cells as well as yeast spheroplasts and nuclei preparations (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).

Immunocytochemistry Analysis: A representative lot immunostained microtubules of fixed Dictyostelium (amoeba) as well as isolated and stabilized amoeba cytoskeleton (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).

Western Blotting Analysis: A representative lot detected α-tubulin in spermatozoa sample from from fertile and infertile men (Breitling, F., et al. (1991). Gene. 104(2):147-153).
Biological Information
ImmunogenYeast tubulin (Kilmartin, J.V., et al. (1982). J. Cell Biol. 93(3):576-582).
CloneYOL1/34
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone YOL1/34 reacts with alpha-tubulin, but not beta-tubulin, by targeting a C-terminal epitope highly conserved among alpha-tubulin subtypes. Epitope is not present in alpha-tubulin 4B (TUBA4B; UniProt Q9H853).
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
  • Porcine
  • Rat
  • Yeast
Species Reactivity NoteHuman, Mouse, Porcine, Rat, Amoeba, Yeast. Exprected to react with a broad range of species based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TUB1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~50 kDa observed. 50.14/46.30 kDa (human alpha-1A isoform 1/2; TUBA1A; TUBA3), 50.15/37.22 kDa (human alpha-1B isoform 1/2; TUBA1B), 44.96 kDa (human alpha-2; TUBA2; TUBA3C; TUBA3D), 49.92/48.33 kDa (human alpha-4A isoform 1/2; TUBA4A; TUBA1), 50.14 kDa (mouse & rat alpha-1A; Tuba1a; Tuba1), 50.15 kDa (mouse & rat alpha-1B; Tuba1b; Tuba2), 49.96 kDa (mouse & rat alpha-3; Tuba3; Tuba3a; Tuba3b), 49.92 kDa (mouse & rat alpha-4A; Tuba4a; Tuba4), 49.80 kDa (yeast) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected alpha-tubulin in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CBL270-I 04054839083631

Documentation

Anti-alpha-Tubulin Antibody, clone YOL1/34 SDS

Title

Safety Data Sheet (SDS) 

Anti-alpha-Tubulin Antibody, clone YOL1/34 Certificates of Analysis

TitleLot Number
Anti-alpha-Tubulin, clone YOL1/34 - 3209748 3209748
Anti-alpha-Tubulin, clone YOL1/34 - 3540892 3540892
Anti-alpha-Tubulin, clone YOL1/34 - 4193544 4193544
Anti-alpha-Tubulin, clone YOL1/34 - 4229871 4229871
Anti-alpha-Tubulin, clone YOL1/34 -Q2764871 Q2764871
Anti-alpha-Tubulin, clone YOL1/34 Monoclonal Antibody 3011478