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MABE1073 Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7

Anti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: SMC3, BAM, BMH, CDLS3, CSPG6, SMC3L1 UniProt Number: Q9UQE7
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MABE1073
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HChIP, ChIP-seq, ELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1073
DescriptionAnti-acetyl SMC3 Antibody (Lys105/106), clone 21A7
Alternate Names
  • Structural maintenance of chromosomes protein 3, Lys105/106 acetylated
  • Bamacan, Lys105/106 acetylated
  • Basement membrane-associated chondroitin proteoglycan, Lys105/106 acetylated
  • Chondroitin sulfate proteoglycan 6, Lys105/106 acetylated
  • Chromosome-associated polypeptide, Lys105/106 acetylated
  • hCAP, Lys105/106 acetylated
  • SMC protein 3, Lys105/106 acetylated
  • SMC-3, Lys105/106 acetylated
Background InformationStructural maintenance of chromosomes protein 3 (UniProt Q9UQE7; also known as Bamacan, Basement membrane-associated chondroitin proteoglycan, Chondroitin sulfate proteoglycan 6, Chromosome-associated polypeptide, hCAP, SMC protein 3, SMC-3) is encoded by the SMC3 (also known as BAM, BMH, CDLS3, CSPG6, SMC3L1) gene (Gene ID 9126) in human. Cohesion is a nuclear tripartite complex composed of Rad21 and two structural maintenance of chromosomes proteins, SMC1 and SMC3. Cohesin plays an important role in sister chromatid cohesion, as well as in DNA repair and gene expression regulation. To establish sister chromatid cohesion during S phase, SMC3 is acetylated (on K105 and K106 in human) by two cohesin acetyltransferases (CoATs), ESCO1 and ESCO2, that differ in their N-terminal domains and expression during development and across the cell cycle. ChIP-seq analysis reveals that ESCO1 is recruited by cohesin to over 11,000 chromatin sites occupied by cohesin and CTCF that mediate long-range chromatin interactions and regulate transcription globally. On ther other hand, ESCO2 is infrequently enriched at sites targeted by REST/NRSF (RE1-silencing transcription factor/neuron-restrictive silencer factor) that represses transcription of neuron-specific genes. SMC3 acetylation is thought to induce conformational changes of the dimeric hinge structure during S phase and to allow dimer opening and loading of DNA.
References
Product Information
FormatPurified
PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-acetyl SMC3 (Lys105/106), clone 21A7, Cat. No. MABE1073, is a highly specific mouse monoclonal antibody that targets SMC3 Lys105/106 acetylation and has been tested in Chromatin Immunoprecipitation (ChIP), ChIP-seq, ELISA, and Western Blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
  • ELISA
  • Western Blotting
Application NotesELISA Analysis: A representative lot bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected enhanced SMC3 occupancy at target chromatin sites in HDAC8-null HCT116 cells than wild-type HCT-116 cells (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).

ChIP-seq Analysis: A representative lot detected SMC3 chromosome 11 enrichment sites, including 81% of Rad21 target sites and 70% of Esco1 target sites, by ChIP-seq (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).

Western Blotting Analysis: A representative lot detected SMC3 Lys105/106 acetylation in HeLa cells. ESCO1 or ESCO2 depletion by shRNA treatment reduced SMC3 Lys105/106 acetylation level, while dual ESCO1/2 depletion resulted in most profound SMC3 Lys105/106 acetylation reduction (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the target region sequence of human/mouse/rat SMC3 containing acetylated Lys105 and Lys106.
Clone21A7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 21A7 bound Lys105/106-diacetylated SMC3 peptide with 2-fold higher affinity than Lys105-monoacetylated SMC3 peptide (Kd = 0.04 and 0.09 µg/mL, respectively), while displaying much reduced affinity toward Lys105-monoacetylated (Kd = 0.73 µg/mL) and little affinity toward nonacetylated (Kd = 2.99 µg/mL) peptide (Rahman, S., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(36):11270-11275).
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Bovine, Mouse, Non-Human Primate, Rat, Xenopus based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SMC3
  • BAM
  • BMH
  • CDLS3
  • CSPG6
  • SMC3L1
Modifications
  • Acetylation
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~160 kDa observed. 142.0/141.5/141.6/138.4/140.7 kDa (bovine/human/mouse/rat/xenopus) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retinal epithelial pigment (REP) cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a reduced SMC3 Lys105/106 acetylation in 10 µg of lysate from N-acetyltransferase ESCO1-knockout human retinal epithelial pigment (REP) cells than in lysate from wild-type REP cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1073 04054839083686

Documentation

Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7 SDS

Title

Safety Data Sheet (SDS) 

Anti-acetyl SMC3 Antibody (Lys105/106), clone 21A7 Certificates of Analysis

TitleLot Number
Anti-acetyl SMC3 (Lys105/106), -Q2767827 Q2767827
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 2895552 2895552
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3068067 3068067
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3244387 3244387
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3540378 3540378
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3703565 3703565
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3794315 3794315
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3850516 3850516
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 3990924 3990924
Anti-acetyl SMC3 (Lys105/106), clone 21A7 - 4123895 4123895
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