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MABT11 Anti-ZO-1 Antibody, clone R40.76

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MABT11
100 µg  
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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RWB, IP, IHCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT11
DescriptionAnti-ZO-1 Antibody, clone R40.76
Alternate Names
  • tight junction protein 1
  • zonula occludens 1 protein
  • Zona occludens protein 1
  • Zonula occludens protein 1
  • zona occludens 1
Background InformationTight junctions are complexes of proteins that create intercellular boundaries between the plasma membrane domains of epithelial and endothelial cells. Many of the tight junction-associated proteins are members of the membrane associated guanylate kinase (MAGUK) family and include occludin and zona occludin family members ZO-1, ZO-2 and ZO-3. These proteins are thought to have both structural and signaling roles, and are characteristically defined by three protein-protein interaction modules: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. ZO-1 forms complexes with either ZO-2 or ZO-3. In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction. ZO-1 expression is significantly reduced in many breast cancer lines. ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue.
References
Product Information
FormatPurified
Control
  • Mouse brain lysate
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ZO-1 Antibody, clone R40.76 detects level of ZO-1 & has been published & validated for use in WB, IP, IH.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
Application NotesImmunofluorescence Analysis: A previous lot was used by an indepenedent laboratory in IF (D'Angelo Siliciano, 1988).
Biological Information
ImmunogenPurified protein corresponding to mouse ZO-1.
EpitopeUnknown
CloneR40.76
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificityThe antibody recognizes ZO-1.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with rat based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ZO-1
  • ZO1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

SUBUNIT STRUCTURE: Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CXADR, GJD3, HSPA4 and UBN1 by similarity. Interacts with occludin, claudins, GJA12 and KIRREL1.

SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side by similarity. Cell junction › tight junction by similarity. Note: Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact by similarity.

DOMAIN: The second PDZ domain mediates interaction with GJA12.

SEQUENCE SIMILARITIES: Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. Contains 1 ZU5 domain.
Molecular Weight~220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western blot in mouse brain lysate.

Western blot Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 10 µg of mouse brain lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT11 04053252410789

Documentation

Anti-ZO-1 Antibody, clone R40.76 SDS

Title

Safety Data Sheet (SDS) 

Anti-ZO-1 Antibody, clone R40.76 Certificates of Analysis

TitleLot Number
Anti-ZO-1, clone R40.76 - 2360626 2360626
Anti-ZO-1, clone R40.76 - 2428729 2428729
Anti-ZO-1, clone R40.76 - 2432121 2432121
Anti-ZO-1, clone R40.76 - 2459059 2459059
Anti-ZO-1, clone R40.76 - 1980397 1980397
Anti-ZO-1, clone R40.76 - 2030039 2030039
Anti-ZO-1, clone R40.76 - 2107280 2107280
Anti-ZO-1, clone R40.76 - 2145113 2145113
Anti-ZO-1, clone R40.76 - 2188554 2188554
Anti-ZO-1, clone R40.76 - 2273847 2273847

References

Reference overviewPub Med ID
FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.
Zhang, X; Martinez, D; Koledova, Z; Qiao, G; Streuli, CH; Lu, P
Development (Cambridge, England)  141  3352-62  2014

Show Abstract
25078648 25078648
Transplantation of human umbilical mesenchymal stem cells cures the corneal defects of mucopolysaccharidosis VII mice.
Coulson-Thomas, VJ; Caterson, B; Kao, WW
Stem cells (Dayton, Ohio)  31  2116-26  2013

Show Abstract
23897660 23897660
Patient-specific iPSC-derived photoreceptor precursor cells as a means to investigate retinitis pigmentosa.
Tucker, BA; Mullins, RF; Streb, LM; Anfinson, K; Eyestone, ME; Kaalberg, E; Riker, MJ; Drack, AV; Braun, TA; Stone, EM
eLife  2  e00824  2013

Show Abstract
23991284 23991284