Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABF62 Anti-Tim3 Antibody, clone 2E2

Anti-Tim3 Antibody, clone 2E2 detects level of Tim3 & has been published & validated for use in WB, FC.

Anti-Tim3 Antibody, clone 2E2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: HAVCR2, TIM3, TIMD3 UniProt Number: Q8TDQ0
View Products on Sigmaaldrich.com
MABF62
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF62
DescriptionAnti-Tim3 Antibody, clone 2E2
Alternate Names
  • Hepatitis A virus cellular receptor 2 homolog
  • T-cell immunoglobulin and mucin domain-containing protein 3
  • T-cell membrane protein 3
  • TIMD-3
  • TIM-3
Background InformationAs a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. It is known to synergize with Toll-like receptors and is thought to regulate Th1 and Th17 cytokine secretion.
References
Product Information
FormatPurified
Control
  • Human thymus tissue lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Tim3 Antibody, clone 2E2 detects level of Tim3 & has been published & validated for use in WB, FC.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A 1:1,000 dilution of this antibody detected Tim3 in human monocyte derived dendritic cells.
Biological Information
ImmunogenRecombinant protein corresponding to human Tim3.
Clone2E2
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.
Gene Symbol
  • HAVCR2
  • TIM3
  • TIMD3
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Molecular Weight~30 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human thymus tissue lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Tim3 in human thymus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF62 04053252591327

Documentation

Anti-Tim3 Antibody, clone 2E2 SDS

Title

Safety Data Sheet (SDS) 

Anti-Tim3 Antibody, clone 2E2 Certificates of Analysis

TitleLot Number
Anti-Tim3, clone 2E2 - 2373432 2373432
Anti-Tim3, clone 2E2 - 2394109 2394109
Anti-Tim3, clone 2E2 - 2420526 2420526
Anti-Tim3, clone 2E2 - 2318833 2318833
Anti-Tim3, clone 2E2 - 2505471 2505471
Anti-Tim3, clone 2E2 - 2906340 2906340
Anti-Tim3, clone 2E2 - 3532430 3532430
Anti-Tim3, clone 2E2 - NRG1906479 NRG1906479
Anti-Tim3, clone 2E2 -2527586 2527586
Anti-Tim3, clone 2E2 -2568153 2568153
Show more

References

Reference overviewPub Med ID
Validation and comparison of tumor-associated trypsin inhibitor (TATI) immunoassays.
Elisabete Janeiro,Joana Guimarães,Ulf-Håkan Stenman,Manuela Catarino,Outi Itkonen,Joana Guimar,Ulf-H Stenman
Clinica chimica acta; international journal of clinical chemistry  413  2012

Show Abstract 		22507084 22507084