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PC176 Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb

This Anti-Serotonin 2A Receptor Rabbit pAb is validated for use in Frozen Sections, WB, Free Floating Sections, Paraffin Sections for the detection of Serotonin 2A Receptor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC176
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
M, R, ShRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC176-100ULCN Glass bottle 100 ul
Description
OverviewRecognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
Catalogue NumberPC176
Brand Family Calbiochem®
Application Data
Detection of rat serotonin 2A receptor by immunohistochemistry. Sample: Rat cortex, amygdala, and hippocampus. Primary antibody: Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb (Cat. No. PC176) (1:500). Detection: fluorescence.
References
ReferencesLeonard, B.E. 1996. Psychother. Psychosom. 65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFree Floating Sections Touri, F., et al. 2004. J. Chem. Neuroanatomy 27, 99. Haszprunar, G., et al. 2002. J. Morphol. 251, 210. Paraffin Sections Jian, B., et al. 2002. Am. J. Pathol. 161, 2111.
Key Applications Free-floating Sections
Frozen Sections
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesFrozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections (see application references)
Paraffin Sections (see application references; heat pre-treatment required)
Application CommentsAntibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
ImmunogenRat
HostRabbit
Species Reactivity
  • Mouse
  • Rat
  • Sheep
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC176-100ULCN 04055977209518

Documentation

Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb Certificates of Analysis

TitleLot Number
PC176

References

Reference overview
Leonard, B.E. 1996. Psychother. Psychosom. 65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-August-2007 JSW
ApplicationFrozen Sections (1:300-1:500)
Immunoblotting (1:500-1:1000)
Free Floating Sections (see application references)
Paraffin Sections (see application references; heat pre-treatment required)
Application Data
Detection of rat serotonin 2A receptor by immunohistochemistry. Sample: Rat cortex, amygdala, and hippocampus. Primary antibody: Anti-Serotonin 2A (5-HT2A) Receptor (22-41) Rabbit pAb (Cat. No. PC176) (1:500). Detection: fluorescence.
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~53 kDa serotonin 2A (5-HT2A) receptor.
BackgroundAt least 14 mammalian serotonin receptor subtypes have been identified and classified into several families on the basis of common structural, pharmacological, and functional criteria. Serotonin (5-hydroxytryptamine [5-HT]) mediates its effects in a number of physiological processes including anxiety, depression, sexual activity, and sleep through interactions with different receptor subtypes. These receptors differ in their tissue and cellular localization, affinity for serotonin, and second messenger pathways. The majority of these receptors stimulate a GTP-binding protein upon agonist stimulation and couple to adenylate cyclase or phospholipase C. In contrast, the serotonin 5-HT3 receptor acts as a cation-selective channel. The serotonin receptors have generated considerable pharmacological interest as targets for the identification of selective drugs that interact with a specific receptor subtype.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide corresponding to amino acids 22-41 of rat 5-HT2A receptor
Speciesmouse, rat, sheep
FormLiquid
FormulationIn 150 mM NaCl, 20 mM sodium phosphate, 1% BSA, pH 7.5.
Preservative≤0.1% sodium azide
CommentsAntibody specificity was examined in the rat amygdala, cortex, and hippocampus. Detection of primary antibody with ABC according to manufacturers directions. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. Addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solutions allows further dilution of the primary antibody to approximately 1:1000 or greater. For staining paraffin sections, heating in 10 mM citrate buffer is required (see application references). Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesLeonard, B.E. 1996. Psychother. Psychosom. 65, 66.
Teitler, M. and Herrick-Davis, K. 1994. Crit. Rev. Neurobiol. 8, 175.
Application referencesFree Floating Sections Touri, F., et al. 2004. J. Chem. Neuroanatomy 27, 99. Haszprunar, G., et al. 2002. J. Morphol. 251, 210. Paraffin Sections Jian, B., et al. 2002. Am. J. Pathol. 161, 2111.