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05-1000X Anti-Phosphoserine Antibody, clone 4A4

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05-1000X
50 µg  
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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
VrtWB, IF, FC, IH(P), ELISAMPurifiedMonoclonal Antibody
Description
Catalogue Number05-1000X
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphoserine Antibody, clone 4A4
Background InformationThe identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotransmission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that the majority of all phosphorylation events occur on serine and threonine residues (greater than 95%).
Product Information
FormatPurified
HS Code3002 15 90
Presentation50 µL of PBS with 0.05% sodium azide with 30% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Phosphoserine Antibody, clone 4A4 is an antibody against Phosphoserine for use in WB, IF, FC, IH(P), ELISA.
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
  • ELISA
Biological Information
ImmunogenPhosphoserine coupled to KLH
Clone4A4
HostMouse
SpecificitySerine-phosphorylated proteins from all species
IsotypeIgG1
Species Reactivity
  • Vertebrates
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G-Sepharose™ Chromatography
Molecular WeightDependent upon the molecular weight of the serine phosphorylated protein being detected.
Product Usage Statements
Quality AssuranceRoutinely evaluated by immunoblot analysis on lysate from Calyculin A/Okadaic-treated human A431 carcinoma cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions2 years at -20°C from date of shipment.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C.
Packaging Information
Material Size50 µg
Global Trade Item Number
Catalogue Number GTIN
05-1000X 04053252366802