Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABS234 Anti-PI3 Kinase Antibody, p85

Detect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.

Anti-PI3 Kinase Antibody, p85 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: Pik3r1 UniProt Number: Q63787
View Products on Sigmaaldrich.com
ABS234
200 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IPRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS234
Replaces06-497
DescriptionAnti-PI3 Kinase Antibody, p85
Alternate Names
  • Phosphatidylinositol 3-kinase regulatory subunit alpha
  • PI3-kinase regulatory subunit alpha
  • PI3K regulatory subunit alpha
  • PtdIns-3-kinase regulatory subunit alpha
  • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha
  • PI3-kinase subunit p85-alpha
  • PtdIns-3-kinase regulatory subunit p85-alpha
Background InformationPhosphatidylinositol 3-Kinase (PI3 Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β,γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibited by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
References
Product Information
FormatAffinity Purified
Control
  • Jurkat cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect PI3 Kinase, p85 using this rabbit polyclonal antibody, Anti-PI3 Kinase Antibody, p85 validated for use in western blotting & IP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated PI3 Kinase, p85 in 0.5 mg of Jurkat cell lysate.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to rat PI3 Kinase, p85.
HostRabbit
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteDemonstrated to react with Human, and Rat. Predicted to react with Mouse, Bovine, and Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Pik3r1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~85 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:600 dilution of this antibody detected PI3 Kinase, p85 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS234 04053252906398

Documentation

Anti-PI3 Kinase Antibody, p85 SDS

Title

Safety Data Sheet (SDS) 

Anti-PI3 Kinase Antibody, p85 Certificates of Analysis

TitleLot Number
Anti-PI3 Kinase p85 - 3201694 3201694
Anti-PI3 Kinase p85 - 3425574 3425574
Anti-PI3 Kinase p85 - 4011209 4011209
Anti-PI3 Kinase p85 Polyclonal Antibody 3153268
Anti-PI3 Kinase, p85 - 2527196 2527196
Anti-PI3 Kinase, p85 - Q2187161 Q2187161
Anti-PI3 Kinase, p85 -2577052 2577052
Anti-PI3 Kinase, p85 -2608853 2608853
Anti-PI3 Kinase, p85 -2625366 2625366
Anti-PI3 Kinase, p85 -2687798 2687798
Show more