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This Anti-Opioid µ Receptor (384-398) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, IF, IP for the detection of Opioid µ Receptor (384-398).
More>>This Anti-Opioid µ Receptor (384-398) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, IF, IP for the detection of Opioid µ Receptor (384-398). Less<<
Anti-Opioid μ Receptor (384-398) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Frozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP) Immunoblotting (1:2000-1:2500; see application references) Immunofluorescence (1:100-1:200) Immunoprecipitation (see comments)
Application Comments
The specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
Immunogen
Rat
Host
Rabbit
Isotype
IgG
Species Reactivity
Rat
Antibody Type
Polyclonal Antibody
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Highly Toxic
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
Anti-Opioid μ Receptor (384-398) Rabbit pAb Certificates of Analysis
Title
Lot Number
PC165L
References
Reference overview
Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol.36, 379. Arvidsson, U., et al. 1995. J. Neurosci.15, 3328. Kieffer, B.L. 1995. Cell. Mol. Neurobiol.15, 615-635. Childers, S.R. 1991. Life Sci.48, 1991.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-October-2008 RFH
Application
Frozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP) Immunoblotting (1:2000-1:2500; see application references) Immunofluorescence (1:100-1:200) Immunoprecipitation (see comments)
Description
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~44-45 kDa opioid µ receptor protein.
Background
Opioid peptides are endogenous neuromodulators, which have been identified from brain, and play a major role in the nociceptive pathway by interacting with several membrane receptors, defined as µ, δ, and κ. These peptides are derived from the cleavage of precursor proteins to produce the β-endorphin, enkephalin, and dynorphin peptides. Leu-enkephalin, Met-enkephalin, and β-endorphin all bind the µ and δ receptors with high affinity, while β-endorphin also exhibits low affinity κ binding. Dynorphin A and dynorphin B both bind the κ receptor with high affinity. In addition to these endogenous peptides, a number of exogenous nonpeptide molecules known as alkaloids or opiates also interact with the receptors to mediate a number of biological events which include the modulation of pain, analgesia, behavior, locomotor activity, and neuroendocrine physiology. Although the δ opioid receptor subtype was the first subtype identified, all three receptor classes are G protein-coupled receptors which have been shown to inhibit adenylate cyclase, decrease the conductance of voltage-gated Ca2+ channels, or activate K+ channel current.
Host
Rabbit
Immunogen species
Rat
Immunogen
a synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor
Isotype
IgG
Species
rat
Positive control
Rat caudate putamen or spinal cord (dorsal horn)
Form
Lyophilized
Formulation
Undiluted serum.
Preservative
None
Comments
The specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).
Toxicity
Highly Toxic
References
Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol.36, 379. Arvidsson, U., et al. 1995. J. Neurosci.15, 3328. Kieffer, B.L. 1995. Cell. Mol. Neurobiol.15, 615-635. Childers, S.R. 1991. Life Sci.48, 1991.
Application references
Immunoblotting
Wang, X., et al. 2004. Neurosci.129, 751.
Immunoblotting, Immunofluorescence, Frozen Sections
Arvidsson, U., et al. 1995. J. Neurosci.15, 3328.
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