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ABN96 Anti-Neurexin3 Antibody

Detect Neurexin3 using this Anti-Neurexin3 Antibody validated for use in WB, IH.

Anti-Neurexin3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: NRXN3, C14orf60, KIAA0743 UniProt Number: Q9Y4C0
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ABN96
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN96
DescriptionAnti-Neurexin3 Antibody
Alternate Names
  • Neurexin-3-alpha
  • Neurexin III-alpha
Background InformationNeurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion. (Missler, M., et al. (1998) 14(1):20-26) Antibody Anti-neurexin 3 alpha (ABN96) was made against a peptide immunogen corresponding to a stretch of amino acids in the extracellular domain of human neurexin 3 alpha (Uniprot Q9Y4C0). Although the theoretical full length of Neurexin 1alpha is 180 kDa, the protein detected in human brain lysates may show a band around 180 kDa and additional bands around 40-55 kDa as a possible result of the posttranslational processing previously reported.
References
Product Information
FormatAffinity Purified
Control
  • PC12 cell lysate.
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Neurexin3 using this Anti-Neurexin3 Antibody validated for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Neurexin-3 in normal mouse brain tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Neurexin-3.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNeurexins are a family of proteins that function in the vertebrate nervous system as cell adhesion molecules and receptors. They are encoded by several unlinked genes of which two, NRXN1 and NRXN3, are among the largest known human genes. Three of the genes (NRXN1-3) utilize two alternate promoters and include numerous alternatively spliced exons to generate thousands of distinct mRNA transcripts and protein isoforms. The majority of transcripts are produced from the upstream promoter and encode alpha-neurexin isoforms; a much smaller number of transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. The alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and have been shown to interact with neurexophilins. The beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins.
Gene Symbol
  • NRXN3
  • C14orf60
  • KIAA0743
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Neuronal cell surface protein that may be involved in cell recognition and cell adhesion. May mediate intracellular signaling.

SUBUNIT STRUCTURE: The laminin G-like domain 2 binds to NXPH1. Specific isoforms bind to alpha-dystroglycan. The cytoplasmic C-terminal region binds to CASK.

SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Molecular Weight~180 kDa observed. UniProtKB/Swiss-Prot Q9Y4C0 (NRX3A_HUMAN) states 2 isoforms due to alternative promoter usage and alternative splicing at a molecular weight of ~181 kDa and ~117 kDa, with several beta isoforms at a molecular weight 70 kDa, 51 kDa, 47 kDa, and 44 kDa. Additional bands may also be observed around 40-55 kDa as a possible result of the posttranslational processing.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Neurexin-3 in 10 µg of PC12 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN96 04053252421181