Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

PC138 Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb

This Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb is validated for use in ELISA, Gel Shift, Immunoblotting, Immunoprecipitation for the detection of NF-κB (p65, RelA) (Ab-2).

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Nuclear Factor ΚB

View Products on Sigmaaldrich.com
PC138
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC138-100ULCN Plastic ampoule 100 ul
Description
OverviewRecognizes the ~65 kDa NF-κB p65 (RelA) protein in Daudi cells.
Catalogue NumberPC138
Brand Family Calbiochem®
SynonymsAnti-Nuclear Factor ΚB
References
ReferencesKolberg, R., 1997. J. NIH Research 9, 29.
Van Antwerp, D.J. et al. 1996. Science 274, 787.
Beg, A.A., et al. 1995. Nature 376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol. 12, 141.
Wulczyn, F.G., et al. 1992. Nature 358, 597.
Product Information
FormLiquid
FormulationIn 5 mM sodium phosphate buffer, pH 7.4.
Positive controlDaudi cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Gel Shift
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
Application CommentsThis antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC138-100ULCN 04055977209464

Documentation

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-NF-κB (p65, RelA) (Ab-2) Rabbit pAb Certificates of Analysis

TitleLot Number
PC138

References

Reference overview
Kolberg, R., 1997. J. NIH Research 9, 29.
Van Antwerp, D.J. et al. 1996. Science 274, 787.
Beg, A.A., et al. 1995. Nature 376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol. 12, 141.
Wulczyn, F.G., et al. 1992. Nature 358, 597.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-October-2007 RFH
SynonymsAnti-Nuclear Factor ΚB
ApplicationELISA (see comments)
Gel Shift (see comments)
Immunoblotting (1:500, chemiluminescence)
Immunoprecipitation (see comments)
DescriptionRabbit polyclonal antibody. Recognizes the ~65 kDa NF-κB p65 (RelA) protein.
BackgroundNFκB (Nuclear factor κB) is a transcription factor that plays crucial roles in gene regulation including those involved in immune and inflammatory responses. Active NFκB is most commonly composed of the heterodimer DNA binding subunits p50 and p65 (RelA). These subunits are members of a multigene family called the rel family. In addition to p50 and RelA, three other proteins are implicated in dimer formation leading to transcriptional activation, p52, c-rel and rel B. Inhibitors to this process exist including another member of the rel family IκBα which binds to c-rel, p65 and relB preventing DNA binding by their homo- or heterodimers. It has recently been shown that NFκB plays a role in the apoptotic process - when RelA, the gene that codes for an NFκB subunit, was knocked out, this lead to the death of mouse embryos through apoptosis of the liver cells. Similarly, it has been shown in a wide range of cells that NFκB which has been inactivated by IκBα were more sensitive to TNF-α-induced apoptosis than control cells. Evidence exists for NFκB playing both anti- and pro-apoptotic roles - it has been suggested that different combinations of subunits may result in different outcomes in terms of cell survival.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids near the N-terminal domain of human NF-κB p65 (RelA), conjugated to KLH
IsotypeIgG
Specieshuman
Positive controlDaudi cells
FormLiquid
FormulationIn 5 mM sodium phosphate buffer, pH 7.4.
Preservative≤0.1% sodium azide
CommentsThis antibody has also been reported to work for ELISA, gel shift, and immunoprecipitation. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKolberg, R., 1997. J. NIH Research 9, 29.
Van Antwerp, D.J. et al. 1996. Science 274, 787.
Beg, A.A., et al. 1995. Nature 376, 167.
Baeuerle, P.A., and Henkel, T., 1994. Ann. Rev. Immunol. 12, 141.
Wulczyn, F.G., et al. 1992. Nature 358, 597.