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ABN174 Anti-MNX1 (HB9) Antibody

Anti-MNX1 (HB9), Cat. No. ABN174, is a rabbit polyclonal antibody that detects MNX1 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-MNX1 (HB9) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Transcription Factors Gene Symbol: MNX1, HLXB9, HB9 UniProt Number: P50219
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ABN174
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN174
DescriptionAnti-MNX1 (HB9) Antibody
Alternate Names
  • Motor neuron and pancreas homeobox protein 1
  • Homeobox protein HB9
Background InformationMotor neuron and pancreas homeobox protein 1 (UniProt: P50219; also known as Homeobox protein HB9) is encoded by the MNX1 (also known as HLXB9) gene (Gene ID: 3110) in human. MNX1 is a transcription factor that is expressed in lymphoid and pancreatic tissues. Its DNA binding homeobox region is localized in amino acids 241-300. It recognizes and binds to the regulatory elements of target genes and negatively modulates transcription. It plays a role in establishing motor neuron identity, in concert with LIM domain transcription factor LMO4. It is involved in negatively modulating transcription of interneuron genes in motor neurons, acting, at least in part, by blocking regulatory sequence interactions of the ISL1-LHX3 complex. It is also involved in pancreas development and function and may play a role in pancreatic cell fate specification. MNX1 is reported to be abnormally overexpressed in cervical cancer and its high expression is correlated with poorer clinicopathologic characteristics. It accelerates cell cycle transition by transcriptionally downregulating p21. It is also a critical regulator for normal pancreas and loss-of-function mutations are known to cause neonatal diabetes. Mutations in MNX1 gene have been linked to Currarino syndrome, an autosomal disorder that is caused by dorsal-ventral patterning defects during embryonic development. Two isoforms of MNX1 have been described that are produced by alternative splicing. (Ref.: Zhu, B., et al. (2020). Front. Oncol. 10; 1307; Schwaller, J. (2019). Haematologica. 104(1); 1-3).
References
Product Information
FormatAffinity Purified
Control
  • PANC-1 cell lysate
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-MNX1 (HB9), Cat. No. ABN174, is a rabbit polyclonal antibody that detects MNX1 and is tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesTested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected MNX1 in Human pancreas and Mouse brain tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal half of Motor neuron and pancreas homeobox protein 1 (MNX1).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Motor neuron and pancreas homeobox protein 1 (MNX1). It targets an epitope within 18 amino acids from the C-terminal half.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Canine, Bovine, Monkey, Feline, Porcine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MNX1
  • HLXB9
  • HB9
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~47 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in PANC-1 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MNX1 in PANC-1 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN174 04053252695797